16SrDNA-Based Detection Technology in Patients with Chronic Pharyngitis to Analyze the Distribution Characteristics of Pharyngeal Bacteria

Shen, Yage1,2; Liu, Chunhan2,3; Luo, Jiao2; Wang, Jianchao2; Zhang, Di2

2022-03-11

10.1155/2022/5186991

Journal of Healthcare Engineering   影响因子和分区

2040-2295

2040-2309

In order to analyze the distribution characteristics of pharyngeal bacteria in patients with chronic pharyngitis (CP) by 16SrDNA-based detection technology, a prospective study is conducted to collect pharyngeal secretion samples from patients diagnosed with CP who are admitted to the Otorhinolaryngology Department of The Third People's Hospital of Shenzhen from May 2021 to September 2021. Among them, 11 cases are chronic simple pharyngitis (CSP), 11 cases are chronic hypertrophic pharyngitis (CHP), and 8 cases are healthy subjects. All samples are detected by the 16SrDNA technique and analyzed by bioinformatics. 55724.64 +/- 1772.80, 53697.73 +/- 2252.19, and 55177.5 +/- 1661.80 optimized sequences are obtained by 16SrDNA sequencing. The alpha diversity analysis of pharyngeal microflora showed that the abundance index SOBS of pharyngeal microflora is upregulated in patients with CHP compared with normal controls (NC), but the diversity index of pharyngeal bacteria in the three groups is not significantly changed, indicating that the abundance of pharyngeal bacteria in the CHP group is increased. The beta diversity analysis of pharyngeal microflora showed that the three groups are similar in structure and composition, and there is no significant statistical difference. The structural difference analysis of pharyngeal flora combined with LEfSe difference analysis showed that at the phylum level, the relative abundance of Spirochaetes and Synergistetes in the CHP group is significantly higher than that in the CSP group. At the genus level, the relative abundance of opportunistic pathogens such as Selenomonas and Campylobacter increased significantly in the CSP group. The relative abundance of Escherichia, Mycoplasma, and Porphyromonas are significantly increased in the CHP group. The abundance of beneficial symbiotic bacteria decreased significantly in patients with CP. The pharyngitis of patients with CP is characterized by an increase in the abundance of pharyngitis, changes in the structure of pharyngitis, a decline in the symbiotic beneficial bacteria, and an increase in the content of opportunistic pathogens, which may be closely related to the onset and development of CP.

SCI ; EI

英语

通讯

Health Care Sciences & Services

Health Care Sciences & Services

WOS:000779486100003

HINDAWI LTD

Web of Science

1.Hebei Univ, Affiliated Hosp, Coll Clin Med, Baoding 071000, Peoples R China
2.Southern Univ Sci & Technol, Dept Otolaryngol, Peoples Hosp Shenzhen 3, Affiliated Hosp 2, Shenzhen 518000, Peoples R China
3.Southern Univ Sci & Technol, Vertigo Clin, Peoples Hosp Shenzhen 3, Affiliated Hosp 2, Shenzhen 518000, Peoples R China

Shen, Yage,Liu, Chunhan,Luo, Jiao,et al. 16SrDNA-Based Detection Technology in Patients with Chronic Pharyngitis to Analyze the Distribution Characteristics of Pharyngeal Bacteria[J]. Journal of Healthcare Engineering,2022,2022.

Shen, Yage,Liu, Chunhan,Luo, Jiao,Wang, Jianchao,&Zhang, Di.(2022).16SrDNA-Based Detection Technology in Patients with Chronic Pharyngitis to Analyze the Distribution Characteristics of Pharyngeal Bacteria.Journal of Healthcare Engineering,2022.

Shen, Yage,et al."16SrDNA-Based Detection Technology in Patients with Chronic Pharyngitis to Analyze the Distribution Characteristics of Pharyngeal Bacteria".Journal of Healthcare Engineering 2022(2022).