Identification of exosomal mRNA, lncRNA and circRNA signatures in an osteoarthritis synovial fluid-exosomal study

Wu, Xiaomin1; Bian, Bin1; Lin, Zhuofeng1; Wu, Chenxi1; Sun, Yuxin1; Pan, Yu1; Dai, Yong2; Lui, Tun Hing3; Zhuang, Tengfei4; Pan, Xiaohua1

2022

10.1016/j.yexcr.2021.112881

EXPERIMENTAL CELL RESEARCH   影响因子和分区

0014-4827

1090-2422

Aims: Osteoarthritis (OA) is a common degenerative disease that is pathologically characterized by destruction of the joint matrix and reduction of articular chondrocytes, resulting in joint deformity and motor dysfunction. However, the molecular mechanisms governing this pathology have not been elucidated to date.& nbsp;Methods: In this study, we determined the expression levels of lncRNAs, circRNAs, and mRNAs extracted from synovial exosomes of OA and control patients. A network of circRNA/lncRNA-miRNA-mRNA interactions was established using MiRanda and TargetScan software to explore OA pathogenesis. The exosomal lncRNA, circRNA and mRNA expression profiles of the OA and control groups were analysed using LC human competing endogenous RNA (ceRNA) microarrays. The differentially expressed genes were analysed to determine their potential roles in the pathogenesis of OA by bioinformatic analysis.& nbsp;Results: There were 52 mRNAs, 196 lncRNAs and 98 circRNAs differentially expressed in synovial exosomes between osteoarthritis synovial and the control group. The final ceRNA network of lncRNAs and circRNAs exhibited a complex interaction between ncRNA and mRNA related to OA pathological mechanisms. An intersection analysis of the ceRNA network showed that 22 miRNAs, 45 lncRNAs, and 34 circRNAs enriched in the PI3K/Akt and autophagy pathways correlated with 7 mRNAs and may play important roles in OA pathological mechanisms.& nbsp;Conclusion: Our work analysed mRNA/lncRNA/circRNA expression and displayed the ceRNA network of lncRNAs and circRNAs to profile the pathogenesis of OA in synovial exosomes. The results of this study may help to elucidate the pathogenesis of OA and may provide important references for further research attempting to identify more effective targets for the diagnosis and therapy of OA.

Osteoarthritis Synovial exosomes Competing endogenous RNA PI3K/AKT pathway Autophagy pathway

SCI

英语

其他

Science and Technology Project of Guangdong Province[2017B090904016] ; Guangdong Natural Science Foundation[2020A1515011107] ; Shenzhen Science and Technology Innovation Council of China[20180309171218911,"JCYJ20190809113815103"] ; Sanming Project of medicine in Shenzhen["SZSM201506020","SZSM202106019"]

Oncology ; Cell Biology

Oncology ; Cell Biology

WOS:000779968900002

ELSEVIER INC

MOLECULAR BIOLOGY & GENETICS

Web of Science

1.Shenzhen Univ, Dept Orthopaed, Affiliated Hosp 2,Peoples Hosp Shenzhen Baoan Dis, Sch Clin Med 2,Southern Med Univ,Clin Med Coll,Gu, Shenzhen, Peoples R China
2.Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Affiliated Hosp 1,Southern Univ Sci & Technol, Shenzhen, Peoples R China
3.North Dist Hosp, Dept Orthopaed & Traumatol, Sheung Shui, Hong Kong, Peoples R China
4.Shenzhen Yspring Technol Co Ltd, Shenzhen, Peoples R China

Wu, Xiaomin,Bian, Bin,Lin, Zhuofeng,et al.

Identification of exosomal mRNA, lncRNA and circRNA signatures in an osteoarthritis synovial fluid-exosomal study

[J]. EXPERIMENTAL CELL RESEARCH,2022,410(1).

Wu, Xiaomin.,Bian, Bin.,Lin, Zhuofeng.,Wu, Chenxi.,Sun, Yuxin.,...&Pan, Xiaohua.(2022).

Identification of exosomal mRNA, lncRNA and circRNA signatures in an osteoarthritis synovial fluid-exosomal study

.EXPERIMENTAL CELL RESEARCH,410(1).

Wu, Xiaomin,et al."

Identification of exosomal mRNA, lncRNA and circRNA signatures in an osteoarthritis synovial fluid-exosomal study

".EXPERIMENTAL CELL RESEARCH 410.1(2022).