CLE42 delays leaf senescence by antagonizing ethylene pathway in Arabidopsis

Zhang, Yi1,2; Tan, Shuya1; Gao, Yuhan3; Kan, Chengcheng1; Wang, Hou-Ling1; Yang, Qi1; Xia, Xinli1; Ishida, Takashi4; Sawa, Shinichiro5; Guo, Hongwei2; Li, Zhonghai1

2022-04-01

10.1111/nph.18154

NEW PHYTOLOGIST   影响因子和分区

0028-646X

1469-8137

Leaf senescence is the final stage of leaf development and is influenced by numerous internal and environmental factors. CLE family peptides are plant-specific peptide hormones that regulate various developmental processes. However, the role of CLE in regulating Arabidopsis leaf senescence remains unclear. Here, we found that CLE42 is a negative regulator of leaf senescence by using a CRISPR/Cas9-produced CLE mutant collection. The cle42 mutant displayed earlier senescence phenotypes, while overexpression of CLE42 delayed age-dependent and dark-induced leaf senescence. Moreover, application of the synthesized 12-amino-acid peptide (CLE42p) also delayed leaf senescence under natural and dark conditions. CLE42 and CLE41/44 displayed functional redundancy in leaf senescence, and the cle41 cle42 cle44 triple mutant displayed more pronounced earlier senescence phenotypes than any single mutant. Analysis of differentially expressed genes obtained by RNA-Seq methodology revealed that the ethylene pathway was suppressed by overexpressing CLE42. Moreover, CLE42 suppressed ethylene biosynthesis and thus promoted the protein accumulation of EBF, which in turn decreased the function of EIN3. Accordingly, mutation of EIN3/EIL1 or overexpression of EBF1 suppressed the earlier senescence phenotypes of the cle42 mutant. Together, our results reveal that the CLE peptide hormone regulates leaf senescence by communicating with the ethylene pathway.

Arabidopsis thaliana CLE EBF EIN3 ethylene leaf senescence

SCI

英语

其他

National Natural Science Foundation of China[32170345,31970196] ; National Key Research and Development Program of China[2019YFA0903904]

Plant Sciences

Plant Sciences

WOS:000786683100001

WILEY

PLANT & ANIMAL SCIENCE

Web of Science

1.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Natl Engn Res Ctr Tree Breeding & Ecol Restorat, Beijing 100083, Peoples R China
2.Southern Univ Sci & Technol SUSTech, Sch Life Sci, Inst Plant & Food Sci, Dept Biol, Shenzhen 518055, Peoples R China
3.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
4.Kumamoto Univ, Int Res Org Adv Sci & Technol IROAST, Kumamoto 8608555, Japan
5.Kumamoto Univ, Grad Sch Sci & Technol, Kumamoto 8608555, Japan

Zhang, Yi,Tan, Shuya,Gao, Yuhan,et al. CLE42 delays leaf senescence by antagonizing ethylene pathway in Arabidopsis[J]. NEW PHYTOLOGIST,2022.

Zhang, Yi.,Tan, Shuya.,Gao, Yuhan.,Kan, Chengcheng.,Wang, Hou-Ling.,...&Li, Zhonghai.(2022).CLE42 delays leaf senescence by antagonizing ethylene pathway in Arabidopsis.NEW PHYTOLOGIST.

Zhang, Yi,et al."CLE42 delays leaf senescence by antagonizing ethylene pathway in Arabidopsis".NEW PHYTOLOGIST (2022).