南方科技大学知识苑(SUSTech KC): Structural insight into UV-B

题名	Structural insight into UV-B–activated UVR8 bound to COP1
作者	Wang，Yidong 1; Wang，Lixia 1; Guan，Zeyuan 1; Chang，Hongfei 1; Ma，Ling 1; Shen，Cuicui 1; Qiu，Liang 1; Yan，Junjie 1; Zhang，Delin 1; Li，Jian2 ; Deng，Xing Wang 2,3; Yin，Ping 1
通讯作者	Deng，Xing Wang; Yin，Ping
发表日期	2022-04-01
DOI	10.1126/sciadv.abn3337
发表期刊	Science Advances 影响因子和分区
ISSN	2375-2548
EISSN	2375-2548
卷号	8 期号:16
摘要	The CONSTITUTIVE PHOTOMORPHOGENIC 1-SUPPRESSOR OF PHYA-105 (COP1-SPA) complex is a central repressor of photomorphogenesis. This complex acts as an E3 ubiquitin ligase downstream of various light signaling transduced from multiple photoreceptors in plants. How the COP1-SPA activity is regulated by divergent light-signaling pathways remains largely elusive. Here, we reproduced the regulation pathway of COP1-SPA in ultraviolet-B (UV-B) signaling in vitro and determined the cryo-electron microscopy structure of UV-B receptor UVR8 in complex with COP1. The complex formation is mediated by two-interface interactions between UV-B-activated UVR8 and COP1. Both interfaces are essential for the competitive binding of UVR8 against the signaling hub component HY5 to the COP1-SPA complex. We also show that RUP2 dissociates UVR8 from the COP1-SPA4-UVR8 complex and facilitates its redimerization. Our results support a UV-B signaling model that the COP1-SPA activity is repressed by UV-B-activated UVR8 and derepressed by RUP2, owing to competitive binding, and provide a framework for studying the regulatory roles of distinct photoreceptors on photomorphogenesis.
相关链接	[Scopus记录]
收录类别	SCI ; EI
语种	英语
重要成果	NI论文
学校署名	通讯
资助项目	Ministry of Science and Technology of the People's Republic of China[2017YFA0503800];Ministry of Science and Technology of the People's Republic of China[2018YFA0507700];Applied Fundamental Research of Wuhan[2019020701011460];Jackson Laboratory[2021hszd010];National Natural Science Foundation of China[31621001];National Natural Science Foundation of China[31722017];National Natural Science Foundation of China[31870753];National Natural Science Foundation of China[32071211];Southern University of Science and Technology[Y01226026];
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000786214100038
出版者	AMER ASSOC ADVANCEMENT SCIENCE
EI入藏号	20221712035802
EI主题词	Electron microscopes ; Electron microscopy ; Vision
Scopus记录号	2-s2.0-85128632024
来源库	Scopus
引用统计	被引频次[WOS]：18
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/333613
专题	南方科技大学-北京大学植物与食品联合研究所生命科学学院生命科学学院_生物系
作者单位	1.National Key Laboratory of Crop Genetic Improvement,Hubei Hongshan Laboratory,Huazhong Agricultural University,Wuhan,430070,China 2.Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes,Institute of Plant and Food Science,School of Life Sciences,Southern University of Science and Technology,Shenzhen,518055,China 3.School of Advanced Agricultural Sciences,School of Life Sciences,State Key Laboratory of Protein and Plant Gene Research,Peking-Tsinghua Center for Life Sciences,Peking University,Beijing,100871,China
通讯作者单位	南方科技大学-北京大学植物与食品联合研究所; 生物系; 生命科学学院
推荐引用方式 GB/T 7714	Wang，Yidong,Wang，Lixia,Guan，Zeyuan,等. Structural insight into UV-B–activated UVR8 bound to COP1[J]. Science Advances,2022,8(16).
APA	Wang，Yidong.,Wang，Lixia.,Guan，Zeyuan.,Chang，Hongfei.,Ma，Ling.,...&Yin，Ping.(2022).Structural insight into UV-B–activated UVR8 bound to COP1.Science Advances,8(16).
MLA	Wang，Yidong,et al."Structural insight into UV-B–activated UVR8 bound to COP1".Science Advances 8.16(2022).