南方科技大学知识苑(SUSTech KC): Four calcium signaling pathway-related genes were upregulated in microcystic adnexal carcinoma: transcriptome analysis and immunohistochemical validation

题名	Four calcium signaling pathway-related genes were upregulated in microcystic adnexal carcinoma: transcriptome analysis and immunohistochemical validation
作者	Yu, Shuaixia 1,2; Wang, Yang 3 ; Tang, Baijie 1,2; Liu, Xiang 1,2; Song, Linhong 1,2; Xu, Gang 1,2; Zhu, Hong 1,2; Sun, Huajun 1,2
通讯作者	Zhu, Hong; Sun, Huajun
发表日期	2022-05-04
DOI	10.1186/s12957-022-02601-6
发表期刊	WORLD JOURNAL OF SURGICAL ONCOLOGY 影响因子和分区
EISSN	1477-7819
卷号	20 期号:1
摘要	Background Microcystic adnexal carcinoma (MAC) is a skin cancer with challenges in diagnosis and management. This study was aimed to detect molecular alterations of MAC and guide its pathologic diagnosis and treatment. Methods We performed transcriptome analysis on 5 MAC and 5 normal skin tissues, identified the differentially expressed genes, and verified them by immunohistochemistry. Results Three hundred four differentially expressed genes (DEGs) in MAC were identified by next-generation transcriptome sequencing, among which 225 genes were upregulated and 79 genes were downregulated. Four genes of the calcium signaling pathway, including calcium voltage-gated channel subunit alpha 1 S (CACNA1S), ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 (ATP2A1), ryanodine receptor 1 (RYR1), and myosin light chain kinase 3 (MYLK3), were upregulated and then been verified by immunohistochemistry. The expression of CACNA1S, ATP2A1, RYR1, and MYLK3 was upregulated in MAC compared with normal sweat glands and syringoma tumor cells and was generally negative in trichoepithelioma and infundibulocystic type basal cell carcinoma. Conclusions The four genes of the calcium signaling pathway were upregulated in MAC at both RNA and protein levels. CACNA1S, ATP2A1, RYR1, and MYLK3 may be new diagnostic molecular markers and therapeutic targets for MAC.
关键词	Microcystic adnexal carcinoma Molecular markers Transcriptome Calcium signaling pathway Diagnostic markers
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	其他
资助项目	Sichuan Provincial People's Hospital Special Fund[2020ZX01]
WOS研究方向	Oncology ; Surgery
WOS类目	Oncology ; Surgery
WOS记录号	WOS:000790755900002
出版者	BMC
Scopus记录号	2-s2.0-85129376308
来源库	Web of Science
引用统计	被引频次[WOS]：6
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/334348
专题	南方科技大学第一附属医院
作者单位	1.Chinese Acad Sci, Sichuan Translat Med Res Hosp, Sichuan Acad Med Sci & Sichuan Prov Peoples Hosp, Dept Pathol, 32,West Second Sect,First Ring Rd, Chengdu 610072, Peoples R China 2.Univ Elect Sci & Technol China, Sichuan Prov Peoples Hosp, Dept Pathol, Chengdu 611731, Peoples R China 3.Jinan Univ, Affiliated Hosp 1, Southern Univ Sci & Technol, Clin Med Coll 2,Dept Pathol,Shenzhen Peoples Hosp, Shenzhen 518020, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Yu, Shuaixia,Wang, Yang,Tang, Baijie,et al. Four calcium signaling pathway-related genes were upregulated in microcystic adnexal carcinoma: transcriptome analysis and immunohistochemical validation[J]. WORLD JOURNAL OF SURGICAL ONCOLOGY,2022,20(1).
APA	Yu, Shuaixia.,Wang, Yang.,Tang, Baijie.,Liu, Xiang.,Song, Linhong.,...&Sun, Huajun.(2022).Four calcium signaling pathway-related genes were upregulated in microcystic adnexal carcinoma: transcriptome analysis and immunohistochemical validation.WORLD JOURNAL OF SURGICAL ONCOLOGY,20(1).
MLA	Yu, Shuaixia,et al."Four calcium signaling pathway-related genes were upregulated in microcystic adnexal carcinoma: transcriptome analysis and immunohistochemical validation".WORLD JOURNAL OF SURGICAL ONCOLOGY 20.1(2022).