The dual-effects of PLGA@MT electrospun nanofiber coatings on promoting osteogenesis at the titanium-bone interface under diabetic conditions

Wang, Zijie1; Chen, Tingting1; Wu, Zimei1; Jiang, Xingzhu1; Hou, Qiaodan1; Miao, Sikai1; Xia, Ruihao1; Wang, Lin1,2

2022-04-01

10.1039/d2tb00120a

Journal of Materials Chemistry B   影响因子和分区

2050-750X

2050-7518

The high failure risk of endosseous titanium implants under diabetes conditions appeals to strengthen the osteointegration on the titanium-bone (Ti-B) interface. Melatonin (MT) is a neurohormone involved in bone homeostasis, which can promote osteogenesis and inhibit ROS overproduction through multiple pathways, but its effects on the Ti-B interface in diabetes remain elusive. The biodegradable poly(lactic-co-glycolic acid) (PLGA) has excellent controlled and sustained release properties, low cytotoxicity, and biocompatibility. Our study fabricated a nanofiber in which MT was encapsulated in PLGA to generate a nanofiber coating on a polydopamine (PDA)-modified titanium surface using electrospinning technology. The surface characteristic showed that MT was fully encapsulated in the PLGA carrier, and PLGA@MT was strongly coupled to the titanium matrix. Furthermore, the PLGA@MT-Ti nanofiber could release MT for at least 30 days. In vitro cellular tests demonstrated that PLGA@MT-Ti directly stimulates osteogenesis on the Ti-B interface by activating the BMP-4/WNT pathway in a dose-dependent manner. The effect of suppressing diabetes-induced ROS overproduction and promoting cell proliferation was not proportional to the content of MT. In vivo experiments revealed that PLGA@MT-Ti screws promoted the bone formation and osteointegration in type 1 diabetes mellitus (T1DM) mice with tibial bone defects. Our findings demonstrate that PLGA@MT-Ti exerted dual effects through activating the BMP-4/WNT pathway and attenuating ROS overproduction to promote osteogenesis and osteointegration at the Ti-B interface, providing a novel strategy to fabricate biomaterial modification and biofunctionalization under diabetic conditions.

SCI ; EI

英语

第一 ; 通讯

National Natural Science Foundation of China[81972045] ; Provincial College Students' Innovation, Entrepreneurship Training Program[S202114325030] ; Guangdong Basic and Applied Basic Research Foundation[2022A1515012373]

Materials Science

Materials Science, Biomaterials

WOS:000790365500001

ROYAL SOC CHEMISTRY

20222712307696

Biocompatibility ; Bone ; Cell proliferation ; Chemical activation ; Coatings ; Mammals ; Nanofibers ; Titanium compounds

Biological Materials and Tissue Engineering:461.2 ; Biology:461.9 ; Immunology:461.9.1 ; Titanium and Alloys:542.3 ; Nanotechnology:761 ; Chemical Reactions:802.2 ; Chemical Products Generally:804 ; Coating Materials:813.2 ; Solid State Physics:933

Web of Science

1.Southern Univ Sci & Technol, Sch Med, Shenzhen 518055, Peoples R China
2.Southern Univ Sci & Technol Hosp, Shenzhen 518055, Peoples R China

Wang, Zijie,Chen, Tingting,Wu, Zimei,et al. The dual-effects of PLGA@MT electrospun nanofiber coatings on promoting osteogenesis at the titanium-bone interface under diabetic conditions[J]. Journal of Materials Chemistry B,2022,10:4020-4030.

Wang, Zijie.,Chen, Tingting.,Wu, Zimei.,Jiang, Xingzhu.,Hou, Qiaodan.,...&Wang, Lin.(2022).The dual-effects of PLGA@MT electrospun nanofiber coatings on promoting osteogenesis at the titanium-bone interface under diabetic conditions.Journal of Materials Chemistry B,10,4020-4030.

Wang, Zijie,et al."The dual-effects of PLGA@MT electrospun nanofiber coatings on promoting osteogenesis at the titanium-bone interface under diabetic conditions".Journal of Materials Chemistry B 10(2022):4020-4030.