| 题名 | VMP1 and TMEM41B are essential for DMV formation during β-coronavirus infection |
| 作者 | Ji，Mingming1; Li，Meng2  ; Sun，Long3,4; Zhao，Hongyu1; Li，Ying5; Zhou，Lulu6; Yang，Zhenni2; Zhao，Xin1; Qu，Wenyan1; Xue，Hanbing2; Zheng，Ze2; Li，Yiming6; Deng，Hongyu3,4 ; Zhao，Yan G.2,7; Ji，Mingming8; Li，Meng9; Sun，Long10,11; Zhao，Hongyu8; Li，Ying12; Zhou，Lulu13; Yang，Zhenni9; Zhao，Xin8; Qu，Wenyan8; Xue，Hanbing9; Zheng，Ze9; Li，Yiming13; Deng，Hongyu10,11; Zhao，Yan G.9,14
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| 通讯作者 | Deng，Hongyu; Zhao，Yan G.; Deng，Hongyu; Zhao，Yan G. |
| 发表日期 | 2022-06-06
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| DOI | |
| 发表期刊 | |
| ISSN | 0021-9525
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| EISSN | 1540-8140
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| 卷号 | 221期号:6 |
| 摘要 | β-coronaviruses reshape host cell endomembranes to form double-membrane vesicles (DMVs) for genome replication and transcription. Ectopically expressed viral nonstructural proteins nsp3 and nsp4 interact to zipper and bend the ER for DMV biogenesis. Genome-wide screens revealed the autophagy proteins VMP1 and TMEM41B as important host factors for SARS-CoV-2 infection. Here, we demonstrated that DMV biogenesis, induced by virus infection or expression of nsp3/4, is impaired in the VMP1 KO or TMEM41B KO cells. In VMP1 KO cells, the nsp3/4 complex forms normally, but the zippered ER fails to close into DMVs. In TMEM41B KO cells, the nsp3-nsp4 interaction is reduced and DMV formation is suppressed. Thus, VMP1 and TMEM41B function at different steps during DMV formation. VMP1 was shown to regulate cross-membrane phosphatidylserine (PS) distribution. Inhibiting PS synthesis partially rescues the DMV defects in VMP1 KO cells, suggesting that PS participates in DMV formation. We provide molecular insights into the collaboration of host factors with viral proteins to remodel host organelles. |
| 相关链接 | [Scopus记录] |
| 收录类别 | |
| 语种 | 英语
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| 重要成果 | NI论文
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| 学校署名 | 通讯
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| 资助项目 | National Natural Science Foundation of China[32170753]
; Chinese Academy of Sciences[XDB37030205]
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| WOS研究方向 | Cell Biology
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| WOS类目 | Cell Biology
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| WOS记录号 | WOS:000868234900001
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| 出版者 | |
| ESI学科分类 | MOLECULAR BIOLOGY & GENETICS
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| Scopus记录号 | 2-s2.0-85130001864
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| 来源库 | Scopus
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| 引用统计 |
被引频次[WOS]：30
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| 成果类型 | 期刊论文 |
| 条目标识符 | http://sustech.caswiz.com/handle/2SGJ60CL/334805 |
| 专题 | 生命科学学院_生物系 生命科学学院 工学院_生物医学工程系 |
| 作者单位 | 1.National Laboratory of Biomacromolecules,CAS Center for Excellence in Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,China 2.Department of Biology,School of Life Sciences,Southern University of Science and Technology,Shenzhen,China 3.CAS Key Laboratory of Infection and Immunity,CAS Center for Excellence in Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,China 4.University of Chinese Academy of Sciences,China 5.Technology Center for Protein Sciences,School of Life Science,Tsinghua University,China 6.Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,China 7.Brain Research Center,Department of Biology,School of Life Sciences,Southern University of Science and Technology,Shenzhen,China 8.National Laboratory of Biomacromolecules,CAS Center for Excellence in Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,China 9.Department of Biology,School of Life Sciences,Southern University of Science and Technology,Shenzhen,China 10.CAS Key Laboratory of Infection and Immunity,CAS Center for Excellence in Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,China 11.University of Chinese Academy of Sciences,China 12.Technology Center for Protein Sciences,School of Life Science,Tsinghua University,China 13.Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,China 14.Brain Research Center,Department of Biology,School of Life Sciences,Southern University of Science and Technology,Shenzhen,China |
| 通讯作者单位 | 生物系; 生命科学学院 |
| 推荐引用方式 GB/T 7714 |
Ji，Mingming,Li，Meng,Sun，Long,et al. VMP1 and TMEM41B are essential for DMV formation during β-coronavirus infection[J]. JOURNAL OF CELL BIOLOGY,2022,221(6).
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| APA |
Ji，Mingming.,Li，Meng.,Sun，Long.,Zhao，Hongyu.,Li，Ying.,...&Zhao，Yan G..(2022).VMP1 and TMEM41B are essential for DMV formation during β-coronavirus infection.JOURNAL OF CELL BIOLOGY,221(6).
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| MLA |
Ji，Mingming,et al."VMP1 and TMEM41B are essential for DMV formation during β-coronavirus infection".JOURNAL OF CELL BIOLOGY 221.6(2022).
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| 条目包含的文件 | 条目无相关文件。 | |||||
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