Measuring the Migration and Biofilm Formation of Various Bacteria

Ma，Yuhao1; Hasan，Zahariah2; Huang，Jia3; Chen，Jun4; Ho，Chun Loong5

2022-05-02

10.3791/63595

Jove-Journal of Visualized Experiments   影响因子和分区

1940-087X

1940-087X

As microbes that thrive in the host body primarily have adaptive abilities that facilitate their survival, methods for classifying and identifying their nature would be beneficial in facilitating their characterization. Currently, most studies focus only on one specific characterization method; however, the isolation and identification of microorganisms from the host is a continuous process and usually requires several combinatorial characterization methods. Herein, we describe methods of identifying the microbial biofilm-forming ability, the microbial respiration state, and their chemotaxis behavior. The methods are used to identify five microbes, three of which were isolated from the bone tissue of Sprague-Dawley (SD) rats (Corynebacterium stationis, Staphylococcus cohnii subsp. urealyticus, and Enterococcus faecalis) and two from the American Type Culture Collection (ATCC)-Staphylococcus aureus ATCC 25923 and Enterococcus faecalis V583. The microbes isolated from the SD rat bone tissue include the gram-positive microbes. These microbes have adapted to thrive under stressful and nutrient-limiting environments within the bone matrix. This article aims to provide the readers with the specific know-how of determining the nature and behavior of the isolated microbes within a laboratory setting.

SCI

英语

第一

National Natural Science Foundation of China's Research Fund for International Young Scientists[22050410270] ; Shenzhen Special Fund for Innovation and Entrepreneurship of Overseas High-level Talents Peacock Team[KQTD20170810111314625] ; Guangdong Innovative and Entrepreneurial Research Team Program[2019ZT08Y191]

Science & Technology - Other Topics

Multidisciplinary Sciences

WOS:000810986100043

JOURNAL OF VISUALIZED EXPERIMENTS

2-s2.0-85130031590

Scopus

1.Department of Biomedical Engineering,Southern University of Science and Technology,
2.Desa Mahkota Form Six College,Ministry of Education Malaysia,Malaysia
3.Department of Critical Care Medicine,Second Affiliated Hospital School of Medicine,Southern University of Science and Technology,
4.Department of Biomedical Engineering, Southern University of Science and Technology;
5.Department of Biomedical Engineering, Southern University of Science and Technology; hejl@sustech.edu.cn

Ma，Yuhao,Hasan，Zahariah,Huang，Jia,et al. Measuring the Migration and Biofilm Formation of Various Bacteria[J]. Jove-Journal of Visualized Experiments,2022(183).

Ma，Yuhao,Hasan，Zahariah,Huang，Jia,Chen，Jun,&Ho，Chun Loong.(2022).Measuring the Migration and Biofilm Formation of Various Bacteria.Jove-Journal of Visualized Experiments(183).

Ma，Yuhao,et al."Measuring the Migration and Biofilm Formation of Various Bacteria".Jove-Journal of Visualized Experiments .183(2022).