Induced Mutation in GmCOP1b Enhances the Performance of Soybean under Dense Planting Conditions

Ji，Ronghuan1,2; Xu，Xinying1; Liu，Jun1; Zhao，Tao1; Li，Hongyu1; Zhai，Jixian2; Liu，Bin1

2022-05-01

10.3390/ijms23105394

International Journal of Molecular Sciences   影响因子和分区

1661-6596

1422-0067

CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) is the key photomorphogenic inhibitor that has been extensively studied in higher plants. Nevertheless, its role has not been documented in the economically important soybean. Here we investigated the functions of two COP1 homologous genes, GmCOP1a and GmCOP1b, by analyzing Gmcop1a and Gmcop1b mutants with indels using CRISPR in soybean. We revealed that, although both genes are required for skotomorphogenesis in the dark, the GmCOP1b gene seems to play a more prominent role than GmCOP1a in promoting stem elongation under normal light conditions. Consistently, the bZIP transcriptional factors STF1/2, which repress stem elongation in soybean, accumulated to the highest level in the Gmcop1a1b double mutant, followed by the Gmcop1b and Gmcop1a mutants. Furthermore, the Gmcop1b mutants showed reduced shade response and enhanced performance under high-density conditions in field trials. Taken together, this study provides essential genetic resources for elucidating functional mechanisms of GmCOP1 and breeding of high yield soybean cultivars for future sustainable agriculture.

photomorphogenesis shade avoidance soybean stem elongation

SCI

英语

其他

National Natural Science Foundation of China[32072091];

Biochemistry & Molecular Biology ; Chemistry

Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary

WOS:000801600400001

MDPI

CHEMISTRY

2-s2.0-85129807797

Scopus

1.The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI),Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing,100081,China
2.Department of Biology,Southern University of Science and Technology,Shenzhen,518055,China

Ji，Ronghuan,Xu，Xinying,Liu，Jun,et al. Induced Mutation in GmCOP1b Enhances the Performance of Soybean under Dense Planting Conditions[J]. International Journal of Molecular Sciences,2022,23(10).

Ji，Ronghuan.,Xu，Xinying.,Liu，Jun.,Zhao，Tao.,Li，Hongyu.,...&Liu，Bin.(2022).Induced Mutation in GmCOP1b Enhances the Performance of Soybean under Dense Planting Conditions.International Journal of Molecular Sciences,23(10).

Ji，Ronghuan,et al."Induced Mutation in GmCOP1b Enhances the Performance of Soybean under Dense Planting Conditions".International Journal of Molecular Sciences 23.10(2022).