Single Small Extracellular Vesicle (sEV) Quantification by Upconversion Nanoparticles

Huang，Guan1; Zhu，Ying1,2,3; Wen，Shihui1; Mei，Haoqi2; Liu，Yongtao1; Wang，Dejiang1; Maddahfar，Mahnaz1; Su，Qian Peter1,2; Lin，Gungun1,4; Chen，Yinghui1,4,5; Jin，Dayong1,4,6

2022

10.1021/acs.nanolett.2c00724

NANO LETTERS   影响因子和分区

1530-6984

1530-6992

Cancer-derived small extracellular vesicles (sEVs) are potential circulating biomarkers in liquid biopsies. However, their small sizes, low abundance, and heterogeneity in molecular makeups pose major technical challenges for detecting and characterizing them quantitatively. Here, we demonstrate a single-sEV enumeration platform using lanthanide-doped upconversion nanoparticles (UCNPs). Taking advantage of the unique optical properties of UCNPs and the background-eliminating property of total internal reflection fluorescence (TIRF) imaging technique, a single-sEV assay recorded a limit of detection 1.8 × 106 EVs/mL, which was nearly 3 orders of magnitude lower than the standard enzyme-linked immunosorbent assay (ELISA). Its specificity was validated by the difference between EpCAM-positive and EpCAM-negative sEVs. The accuracy of the UCNP-based single-sEV assay was benchmarked with immunomagnetic-beads flow cytometry, showing a high correlation (R2> 0.99). The platform is suitable for evaluating the heterogeneous antigen expression of sEV and can be easily adapted for biomarker discoveries and disease diagnosis.

Cancer Diagnostics Extracellular Vesicles (EVs) Imaging Upconversion Nanoparticles (UCNPs)

SCI ; EI

英语

NI论文

通讯

Oversea Study Program of Guangzhou Elite Project[JY201840] ; Pankind, the Australian Pancreatic Cancer Foundation[APCF R5 2019] ; NHMRC Fellowship[GNT1160635] ; Australian Research Council (ARC) Discovery Early Career Researcher Award Scheme[DE220100846] ; National Natural Science Foundation of China (NSFC)[61729501] ; Major International (Regional) Joint Research Project of NSFC[51720105015] ; Science and Technology Innovation Commission of Shenzhen[KQTD20170810110913065] ; Australia China Science and Research Fund Joint Research Centre for Point-of-Care Testing["ACSRF658277","SQ2017YFGH001190"] ; Australian Research Council Laureate Fellowship Program[FL210100180]

Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics

Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter

WOS:000801231700035

AMER CHEMICAL SOC

20222012119448

Biomarkers ; Diagnosis ; Diseases ; Nanoparticles

Medicine and Pharmacology:461.6 ; Light/Optics:741.1 ; Nanotechnology:761 ; Solid State Physics:933

MATERIALS SCIENCE

2-s2.0-85130004066

Scopus

1.Institute for Biomedical Materials and Devices (IBMD),Faculty of Science,University of Technology Sydney,Sydney,2007,Australia
2.School of Biomedical Engineering,Faculty of Engineering and Information Technology,University of Technology Sydney,Sydney,2007,Australia
3.St. George and Sutherland Clinical School,University of New South Wales Sydney,Sydney,2052,Australia
4.ARC Research Hub for Integrated Device for End-User Analysis at Low Levels,Faculty of Science,University of Technology Sydney,Sydney,2007,Australia
5.Alcolizer Technology Pty Ltd,Perth,36 Mumford Place Balcatta,6021,Australia
6.UTS-SUStech Joint Research Centre for Biomedical Materials and Devices,Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,Guangdong,518055,China

Huang，Guan,Zhu，Ying,Wen，Shihui,et al. Single Small Extracellular Vesicle (sEV) Quantification by Upconversion Nanoparticles[J]. NANO LETTERS,2022,22(9).

Huang，Guan.,Zhu，Ying.,Wen，Shihui.,Mei，Haoqi.,Liu，Yongtao.,...&Jin，Dayong.(2022).Single Small Extracellular Vesicle (sEV) Quantification by Upconversion Nanoparticles.NANO LETTERS,22(9).

Huang，Guan,et al."Single Small Extracellular Vesicle (sEV) Quantification by Upconversion Nanoparticles".NANO LETTERS 22.9(2022).