lncRNA NEAT1 promotes autophagy of neurons in mice by impairing miR-107-5p

Dong, Li1,2; Zheng, Yumin2; Luo, Xiaoguang3

2022-05-02

10.1080/21655979.2022.2062989

Bioengineered   影响因子和分区

2165-5979

2165-5987

This work focused on the exploration of NEAT1 in Parkinson's disease (PD) and aimed to explore its effects on PD and related molecular mechanisms. Two experimental models were initially constructed, including MPTP-induced mice in vivo and the MPP+-induced SH-SY5Y cell line in vitro. Immunofluorescence assays were conducted to determine the TH+ positive cell rate. Pole tests and rotarod tests were also performed for the visualization of behavioral changes in mice. Cellular apoptosis was determined using MTT and flow cytometry assays. Changes in the number of autophagosomes were obtained under a transmission electron microscope. The content of dopamine was confirmed by high performance liquid chromatography. The targeted interrelationship between miR-107-5p and NEAT1 was clarified via dual-luciferase reporter gene assays. Meanwhile, mRNA and protein expressions were also detected using qRT-PCR and Western blot respectively. Furthermore, the level of NEAT1 was positively correlated with MPP+ concentration. Interfering with NEAT1 in the present study promoted cellular proliferation and mediated SH-SY5Y cell apoptosis and autophagy treated with MPP+. An increase was discovered in TH positive neurons and suppressive autophagy in PD mice. miR-107-5p was then considered as a NEAT1 putative target involving apoptosis and autophagy of SH-SY5Y cells. Interfering with NEAT1 efficiently facilitated the viability of SH-SY5Y cells and drastically suppressed autophagy and apoptosis of PD mice induced by MPTP- via elevating miR-107-5p level, which indicated that lncRNA NEAT1 acted as a latent therapeutic factor for PD treatment.

Parkinson's disease autophagy NEAT1 miR-107-5p

SCI

英语

通讯

National Natural Science Project of China[81771375] ; Zhangjiakou Key R&D Program for Big Health and Biomedicine[1921080D]

Biotechnology & Applied Microbiology

Biotechnology & Applied Microbiology

WOS:000797805200001

TAYLOR & FRANCIS INC

Web of Science

1.China Med Univ, Affiliated Hosp 4, Shenyang, Peoples R China
2.China Med Univ, Affiliated Hosp 1, Shenyang, Peoples R China
3.Jinan Univ, Clin Med Coll 2, Shenzhen Peoples Hosp, Affiliated Hosp 1,South Univ Sci & Technol, 1017 Dongmen North Rd, Shenzhen 518020, Peoples R China

Dong, Li,Zheng, Yumin,Luo, Xiaoguang. lncRNA NEAT1 promotes autophagy of neurons in mice by impairing miR-107-5p[J]. Bioengineered,2022,13(5).

Dong, Li,Zheng, Yumin,&Luo, Xiaoguang.(2022).lncRNA NEAT1 promotes autophagy of neurons in mice by impairing miR-107-5p.Bioengineered,13(5).

Dong, Li,et al."lncRNA NEAT1 promotes autophagy of neurons in mice by impairing miR-107-5p".Bioengineered 13.5(2022).