南方科技大学知识苑(SUSTech KC): Lupeol triggers oxidative stress, ferroptosis, apoptosis and restrains inflammation in nasopharyngeal carcinoma via AMPK/NF-kappa B pathway

题名	Lupeol triggers oxidative stress, ferroptosis, apoptosis and restrains inflammation in nasopharyngeal carcinoma via AMPK/NF-kappa B pathway
作者	Zhou, Jing-Chun 1; Wu, Bin 1; Zhang, Jing-Jing 2; Zhang, Wei1
通讯作者	Zhou, Jing-Chun
发表日期	2022-05-01
DOI	10.1080/08923973.2022.2072328
发表期刊	IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY 影响因子和分区
ISSN	0892-3973
EISSN	1532-2513
摘要	["Objective: Nasopharyngeal carcinoma is a malignant tumor with high incidence in Asia. This study investigated the anti-tumor capacities of lupeol in nasopharyngeal carcinoma.","Methods: CCK-8 assay was employed to select the suitable concentration and intervention time of lupeol in 5-8 F and CNE1 cells. The anti-cancer impacts of lupeol were evaluated by flow cytometry, ROS generation, western blotting, ELISA, iron assay, lipid peroxidation, mitochondrial membrane potential (MMP), TUNEL, and immunohistochemistry assays. Additionally, levels of AMPK/NF-kappa B pathway-related proteins were tested by western blotting.","Results: Cell viability was notably decreased after administration of lupeol >= 20 mu M. 20 mu M and 40 mu M lupeol induced cell apoptosis, enhanced oxidative stress and restrained immune response in nasopharyngeal carcinoma cells to some extent, as evidenced by the elevation of apoptotic rate, Bax and cleaved caspase-3 expression, ROS production and malondialdehyde level, and reduction of levels of Bcl-2, MMP, superoxide dismutase, TNF-alpha, IL-6 and IL-1 beta. Also, lupeol promoted the iron secretion and lipid peroxidation, the effects of which were reversed by ferroptosis inhibitor (Fer-1). The inhibitory impacts of lupeol at the doses of 20 mu M and 40 mu M on glutathione and GPX4 levels were observed. Importantly, lupeol significantly elevated AMPK alpha phosphorylation, and reduced the levels of p-I kappa B alpha and nuclear NF-kappa B p65. Rescue assay stated that siAMPK could neutralize the above impacts of lupeol. Moreover, lupeol suppressed tumorigenesis of xenografts in nude mice.","Conclusion: Lupeol exerted the anti-cancer impacts by inducing oxidative stress, ferroptosis and apoptosis, and suppressing inflammation via the AMPK/NF-kappa B pathway in nasopharyngeal carcinoma."]
关键词	Lupeol oxidative stress ferroptosis apoptosis AMPK/NF-kappa B pathway nasopharyngeal carcinoma
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	第一 ; 通讯
资助项目	Science, Technology & Innovation Commission of Shenzhen Municipality[JCYJ20180305163939399]
WOS研究方向	Immunology ; Pharmacology & Pharmacy ; Toxicology
WOS类目	Immunology ; Pharmacology & Pharmacy ; Toxicology
WOS记录号	WOS:000796420900001
出版者	TAYLOR & FRANCIS LTD
ESI学科分类	IMMUNOLOGY
来源库	Web of Science
引用统计	被引频次[WOS]：9
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/335019
专题	南方科技大学第一附属医院
作者单位	1.Southern Univ Sci & Technol, Jinan Univ, Affiliated Hosp 1, Second Clin Med Coll 2,Shenzhen Peoples Hosp,Dept, 1017 Dongmen North Rd, Shenzhen 518020, Guangdong, Peoples R China 2.Peking Univ, Dept Otorhinolaryngol, Shenzhen Hosp, Shenzhen, Peoples R China
第一作者单位	南方科技大学第一附属医院
通讯作者单位	南方科技大学第一附属医院
第一作者的第一单位	南方科技大学第一附属医院
推荐引用方式 GB/T 7714	Zhou, Jing-Chun,Wu, Bin,Zhang, Jing-Jing,et al. Lupeol triggers oxidative stress, ferroptosis, apoptosis and restrains inflammation in nasopharyngeal carcinoma via AMPK/NF-kappa B pathway[J]. IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY,2022.
APA	Zhou, Jing-Chun,Wu, Bin,Zhang, Jing-Jing,&Zhang, Wei.(2022).Lupeol triggers oxidative stress, ferroptosis, apoptosis and restrains inflammation in nasopharyngeal carcinoma via AMPK/NF-kappa B pathway.IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY.
MLA	Zhou, Jing-Chun,et al."Lupeol triggers oxidative stress, ferroptosis, apoptosis and restrains inflammation in nasopharyngeal carcinoma via AMPK/NF-kappa B pathway".IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY (2022).