The Airway Microbiome and Metabolome in Preterm Infants: Potential Biomarkers of Bronchopulmonary Dysplasia

Xu, Qi1,2; Yu, Jialin1; Liu, Dong3; Tan, Qi4; He, Yu4

2022-05-10

10.3389/fped.2022.862157

Frontiers in Pediatrics   影响因子和分区

2296-2360

ObjectivesWe investigated the genomic and metabolic characteristics of the airway microbiome in mild, moderate, severe, and non-bronchopulmonary dysplasia (BPD) preterm infants and explored possible mechanisms underlying BPD. MethodsTwenty-eight preterm infants with gestational age <= 34 weeks and intubated within 24 h after birth were enrolled. According to the severity of BPD, the patients were divided into mild, moderate and severe BPD groups, and the non-BPD group was the control group. Tracheal aspirates (TA) were obtained at intubation and on day 7 after birth. The bacterium in the aspirates were sequenced by 16S rRNA, and the metabolomics of the aspirates were identified by high performance liquid chromatography-quadrupole time of flight mass spectrometry (UHPLC-Q-TOF/MS). The correlation between the differential metabolite and differential bacteria was investigated using Pearson's correlation coefficient corrected for gestational age and birth weight and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. ResultsThere were significant differences in the diversity and composition of airway microbiome and metabolome between severe, moderate and mild BPD and non-BPD premature infants. At birth (day 1), the difference was more pronounced than at day 7. The diversity of airway microbial community decreased, the abundance of Stenotrophomonas increased, and the increased level of sn-glycerol 3-phosphoethanolamine was positively correlated with the severity of BPD. There was a significant positive correlation between the abundance of Stenotrophomonas and the level of sn-glycerol 3-phosphoethanolamine. ConclusionDecreased diversity of the airway microbiome, increased abundance of Stenotrophomonas, and increased level of sn-glycerol 3-phosphoethanolamine may have potential as biomarkers for BPD. The occurrence and severity of BPD are closely related to Stenotrophomonas, which may influence the composition of the lower airway microbiome through its metabolite sn-glycerol 3-phosphoethanolamine, and may be the triggering factor of the disease. The causal relationship needs further study.

BPD microbiome genomics metabonomic biomarkers

SCI

英语

第一 ; 通讯

Pediatrics

Pediatrics

WOS:000799592800001

FRONTIERS MEDIA SA

Web of Science

1.Southern Univ Sci & Technol Hosp, Dept Pediat, Shenzhen, Peoples R China
2.Childrens Hosp, Capital Inst Pediat, Beijing, Peoples R China
3.Shenzhen Peoples Hosp, Dept Neonatol, Shenzhen, Peoples R China
4.Chongqing Med Univ, Childrens Hosp, Dept Neonatol, Chongqing, Peoples R China

Xu, Qi,Yu, Jialin,Liu, Dong,et al. The Airway Microbiome and Metabolome in Preterm Infants: Potential Biomarkers of Bronchopulmonary Dysplasia[J]. Frontiers in Pediatrics,2022,10.

Xu, Qi,Yu, Jialin,Liu, Dong,Tan, Qi,&He, Yu.(2022).The Airway Microbiome and Metabolome in Preterm Infants: Potential Biomarkers of Bronchopulmonary Dysplasia.Frontiers in Pediatrics,10.

Xu, Qi,et al."The Airway Microbiome and Metabolome in Preterm Infants: Potential Biomarkers of Bronchopulmonary Dysplasia".Frontiers in Pediatrics 10(2022).