Cryo-ET detects bundled triple helices but not ladders in meiotic budding yeast

Ma, Olivia X.1,2,9; Chong, Wen Guan1,2; Lee, Joy K. E.1,2; Cai, Shujun1,2; Siebert, C. Alistair3; Howe, Andrew3; Zhang, Peijun3,4; Shi, Jian1,2; Surana, Uttam5,6,7,8; Gan, Lu1,2

2022

10.1371/journal.pone.0266035

PLoS One   影响因子和分区

1932-6203

In meiosis, cells undergo two sequential rounds of cell division, termed meiosis I and meiosis II. Textbook models of the meiosis I substage called pachytene show that nuclei have conspicuous 100-nm-wide, ladder-like synaptonemal complexes and ordered chromatin loops. It remains unknown if these cells have any other large, meiosis-related intranuclear structures. Here we present cryo-ET analysis of frozen-hydrated budding yeast cells before, during, and after pachytene. We found no cryo-ET densities that resemble dense ladder-like structures or ordered chromatin loops. Instead, we found large numbers of 12-nm-wide triple-helices that pack into ordered bundles. These structures, herein called meiotic triple helices (MTHs), are present in meiotic cells, but not in interphase cells. MTHs are enriched in the nucleus but not enriched in the cytoplasm. Bundles of MTHs form at the same timeframe as synaptonemal complexes (SCs) in wild-type cells and in mutant cells that are unable to form SCs. These results suggest that in yeast, SCs coexist with previously unreported large, ordered assemblies.

SCI

英语

其他

UK's national Electron Bio-imaging Centre (eBIC)[BI23297] ; Ministry of Education - Singapore (MOE)["R-154-000-A49-114","R-154000-B42-114","MOE2019-T2-2-045"]

Science & Technology - Other Topics

Multidisciplinary Sciences

WOS:000795453600036

PUBLIC LIBRARY SCIENCE

Web of Science

1.Natl Univ Singapore, Dept Biol Sci, Singapore, Singapore
2.Natl Univ Singapore, Ctr BioImaging Sci, Singapore, Singapore
3.Diamond Light Source Ltd, Harwell Sci & Innovat Campus, Didcot, Oxon, England
4.Univ Oxford, Wellcome Ctr Human Genet, Div Struct Biol, Oxford, England
5.ASTAR, Inst Mol & Cell Biol, Singapore, Singapore
6.ASTAR, Bioproc Technol Inst, Singapore, Singapore
7.ASTAR, Biotransformat Innovat Platform, Singapore, Singapore
8.Natl Univ Singapore, Dept Pharmacol, Singapore, Singapore
9.Southern Univ Sci & Technol, Shenzhen, Peoples R China

Ma, Olivia X.,Chong, Wen Guan,Lee, Joy K. E.,et al. Cryo-ET detects bundled triple helices but not ladders in meiotic budding yeast[J]. PLoS One,2022,17(4).

Ma, Olivia X..,Chong, Wen Guan.,Lee, Joy K. E..,Cai, Shujun.,Siebert, C. Alistair.,...&Gan, Lu.(2022).Cryo-ET detects bundled triple helices but not ladders in meiotic budding yeast.PLoS One,17(4).

Ma, Olivia X.,et al."Cryo-ET detects bundled triple helices but not ladders in meiotic budding yeast".PLoS One 17.4(2022).