Global Quantification of Glutathione S-Transferases in Human Serum Using LC-MS/MS Coupled with Affinity Enrichment

Zhang, Yuxing1,2,3,4,5; Huang, Deliang6; Lv, Ning7; Zhu, Guilan3; Peng, Jinghan6; Chou, Tiansheng3; Zhu, Zhibin6; Wang, Ju3; Chen, Yuanyuan6; Fang, Xiangdong1,2,4,5; Qu, Jiuxin7; Chen, Jun6; Liu, Siqi3

2022-05-06

10.1021/acs.jproteome.2c00049

JOURNAL OF PROTEOME RESEARCH   影响因子和分区

1535-3893

1535-3907

The members of the glutathione S-transferase (GST) superfamily often exhibit functional overlap and can compensate for each other. Their concentrations in serum are considered as disease biomarkers. A global and quantitative evaluation of serum GSTs is therefore urgent, but there is a lack of efficient approaches due to technological limitations. GSH magnetic beads were examined for their affinity to enrich GSTs in serum, and the enriched GSTs were quantitatively targeted using a Q Exactive HF-X mass spectrometer in parallel reaction monitoring (PRM) mode. To optimize the quantification of GST peptides, sample types, trypsin digestion, and serum loading were carefully assessed; a biosynthetic method was employed to generate isotope-labeled GST peptides, and instrumental parameters were systematically optimized. A total of 134 clinical sera were collected for GST quantification from healthy donors and patients with four liver diseases. Using the new approach, GSTs in healthy sera were profiled: 14 GST peptides were quantified, and the abundance of five GST families was ranked GSTM > GSTP > GSTA > MGST1 > GSTT1, ranging from 0.1 to 4 pmol/L. Furthermore, combining the abundance of multiple GST peptides could effectively distinguish different types of liver diseases. Quantification of serum GSTs through targeted proteomics, therefore, has apparent clinical potential for disease diagnosis.

quantitative proteomics GSH affinity glutathione S-transferases human serum liver diseases

SCI

英语

通讯

National Key Research and Development Program of China["2017YFC0906702","2017YFC0906703"] ; Guangdong Basic and Applied Basic Research Foundation[2020A1515010586] ; Science and Technology Program of Shenzhen[JCYJ20190809144005609]

Biochemistry & Molecular Biology

Biochemical Research Methods

WOS:000797404600010

AMER CHEMICAL SOC

BIOLOGY & BIOCHEMISTRY

Web of Science

1.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
2.Univ Chinese Acad Sci, Sino Danish Coll, Beijing 100049, Peoples R China
3.BGI Shenzhen, Shenzhen 518083, Peoples R China
4.Chinese Acad Sci, Beijing Inst Genom, Beijing 100101, Peoples R China
5.Chinese Acad Sci, China Natl Ctr Bioinformat, Beijing 100101, Peoples R China
6.Southern Univ Sci & Technol, Affiliated Hosp 2, Dept Liver Dis, Peoples Hosp Shenzhen 3, Shenzhen 518100, Peoples R China
7.Southern Univ Sci & Technol, Dept Clin Lab, Affiliated Hosp 2, Peoples Hosp Shenzhen 3,Natl Clin Res Ctr Infect, Shenzhen 518114, Peoples R China

Zhang, Yuxing,Huang, Deliang,Lv, Ning,et al. Global Quantification of Glutathione S-Transferases in Human Serum Using LC-MS/MS Coupled with Affinity Enrichment[J]. JOURNAL OF PROTEOME RESEARCH,2022,21(5).

Zhang, Yuxing.,Huang, Deliang.,Lv, Ning.,Zhu, Guilan.,Peng, Jinghan.,...&Liu, Siqi.(2022).Global Quantification of Glutathione S-Transferases in Human Serum Using LC-MS/MS Coupled with Affinity Enrichment.JOURNAL OF PROTEOME RESEARCH,21(5).

Zhang, Yuxing,et al."Global Quantification of Glutathione S-Transferases in Human Serum Using LC-MS/MS Coupled with Affinity Enrichment".JOURNAL OF PROTEOME RESEARCH 21.5(2022).