南方科技大学知识苑(SUSTech KC): Establishment and characterization of a new cell culture system for hepatitis B virus replication and infection

题名	Establishment and characterization of a new cell culture system for hepatitis B virus replication and infection
作者	Song，Yingying 1; Shou，Shuyu 1; Guo，Huimin 1,2,5; Gao，Zixiang 1; Liu，Nannan 1; Yang，Yang 1; Wang，Feifei 1; Deng，Qiang 1; Liu，Jing 1,4; Xie，Youhua 1,3
通讯作者	Liu，Jing
发表日期	2022
DOI	10.1016/j.virs.2022.05.002
发表期刊	VIROLOGICA SINICA 影响因子和分区
ISSN	1674-0769
EISSN	1995-820X
卷号	37 期号:4
摘要	Hepatitis B virus (HBV) is a primary cause of chronic liver diseases in humans. HBV infection exhibits strict host and tissue tropism. HBV core promoter (Cp) drives transcription of pregenomic RNA (pgRNA) and plays a key role in the viral life cycle. Hepatocyte nuclear factor 4α (HNF4α) acts as a major transcriptional factor that stimulates Cp. In this work, we reported that BEL7404 cell line displayed a high efficiency of DNA transfection and high levels of HBV antigen expression after transfection of HBV replicons without prominent viral replication. The introduction of exogenous HNF4α and human sodium taurocholate cotransporting polypeptide (hNTCP) expression into BEL7404 made it permissive for HBV replication and susceptible to HBV infection. BEL7404-derived cell lines with induced HBV permissiveness and susceptibility were constructed by stable co-transfection of hNTCP and Tet-inducible HNF4α followed by limiting dilution cloning. HBV replication in such cells was sensitive to inhibition by nucleotide analog tenofovir, while the infection was inhibited by HBV entry inhibitors. This cell culture system provides a new and additional tool for the study of HBV replication and infection as well as the characterization of antiviral agents.
关键词	BEL7404 Doxycycline (DOX) Hepatitis B virus (HBV) Hepatocyte nuclear factor 4α (HNF4α) Sodium-taurocholate cotransporting polypeptide (NTCP) Tetracycline (Tet)-On
相关链接	[Scopus记录]
收录类别	SCI
语种	英语
学校署名	其他
资助项目	National Natural Science Foundation of China[81971921];National Natural Science Foundation of China[81971931];
WOS研究方向	Virology
WOS类目	Virology
WOS记录号	WOS:000848542000010
出版者	KEAI PUBLISHING LTD
ESI学科分类	MICROBIOLOGY
Scopus记录号	2-s2.0-85130814349
来源库	Scopus
引用统计	被引频次[WOS]：5
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/335502
专题	南方科技大学医学院南方科技大学第二附属医院
作者单位	1.Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS) and Department of Medical Microbiology and Parasitology,School of Basic Medical Sciences,Shanghai Institute of Infectious Diseases and Biosecurity,Shanghai Medical College,Fudan University,Shanghai,200032,China 2.Institute for Hepatology,National Clinical Research Center for Infectious Disease,Shenzhen Third People's Hospital,Shenzhen,518112,China 3.Children's Hospital,Fudan University,Shanghai,201102,China 4.Shanghai Key Laboratory of Medical Epigenetics,Institutes of Biomedical Sciences,School of Basic Medical Sciences,Shanghai Medical College,Fudan University,Shanghai,200032,China 5.The Second Affiliated Hospital,School of Medicine,Southern University of Science and Technology,Shenzhen,518112,China
推荐引用方式 GB/T 7714	Song，Yingying,Shou，Shuyu,Guo，Huimin,et al. Establishment and characterization of a new cell culture system for hepatitis B virus replication and infection[J]. VIROLOGICA SINICA,2022,37(4).
APA	Song，Yingying.,Shou，Shuyu.,Guo，Huimin.,Gao，Zixiang.,Liu，Nannan.,...&Xie，Youhua.(2022).Establishment and characterization of a new cell culture system for hepatitis B virus replication and infection.VIROLOGICA SINICA,37(4).
MLA	Song，Yingying,et al."Establishment and characterization of a new cell culture system for hepatitis B virus replication and infection".VIROLOGICA SINICA 37.4(2022).