南方科技大学知识苑(SUSTech KC): Tendon microstructural disruption promotes tendon-derived stem cells to express chondrogenic genes by activating endoplasmic reticulum stress

题名	Tendon microstructural disruption promotes tendon-derived stem cells to express chondrogenic genes by activating endoplasmic reticulum stress
作者	Liu，Chang 1,2 ; Li，Tian Yu 1; Chen，Yong 1 ; Yang，Huan Huan1 ; Sun，Yu Long 1
发表日期	2022
DOI	10.1002/jor.25362
发表期刊	JOURNAL OF ORTHOPAEDIC RESEARCH 影响因子和分区
ISSN	0736-0266
EISSN	1554-527X
卷号	41 期号:2
摘要	The erroneous differentiation of tendon-derived stem cells (TDSCs) into adipocytes, chondrocytes, and osteoblasts is believed to play an important role in the development of tendinopathy. However, the regulatory mechanisms of TDSC differentiation remain unclear. The aim of this study is to investigate the contribution and mechanism of the tendon microstructural disruption to the differentiation of TDSCs. Bovine Achilles tendons were sliced. The tendon slices were stretched with different tensile strains to mimic the tendon structure alteration at various scales. The TDSCs were cultured on the tendon slices. The differentiation of TDSCs and endoplasmic reticulum (ER) stress in the TDSCs were investigated with quantitative reverse transcription polymerase chain reaction, immunostaining and western blot. The effect of ER stress inhibition on chondrogenic differentiation of the TDSCs was further investigated. The structural alteration did not affect the viability of TDSCs. However, the structural alteration of tendon slices with 6.4% strain promoted TDSCs to express the chondrogenic genes. ER stress-related markers, ATF-4 and PERK, were also upregulated. With the inhibition of ER stress, the expression of ATF-4 and the chondrogenic gene SOX9 of TDSCs were inhibited. The study indicated that tendon microdamage could induce the chondrogenic differentiation of TDSCs through triggering ER stress to activate ATF-4 and SOX9 subsequently.
关键词	chondrogenic differentiation endoplasmic reticulum stress microstructural disruption tendon-derived stem cells
相关链接	[Scopus记录]
收录类别	SCI
语种	英语
学校署名	第一
资助项目	National Natural Science Foundation of China[81871827] ; Science and Technology Innovation Commission of Shenzhen["JCYJ20200109144229150","ZDSYS20200811143752005"] ; Sanming Project of Medicine in Shenzhen[SZSM202111015] ; Shenzhen Key Medical Discipline Construction Fund[SZXK024]
WOS研究方向	Orthopedics
WOS类目	Orthopedics
WOS记录号	WOS:000800863400001
出版者	WILEY
ESI学科分类	CLINICAL MEDICINE
Scopus记录号	2-s2.0-85130707419
来源库	Scopus
引用统计	被引频次[WOS]：3
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/335509
专题	南方科技大学第一附属医院
作者单位	1.Shenzhen Key Laboratory of Musculoskeletal Tissue Reconstruction and Function Restoration,Division of Hand and Microvascular Surgery,Department of Orthopedic Surgery,Shenzhen People's Hospital (the First Affiliated Hospital,Southern University of Science and Technology the Second Clinical Medical College,Jinan University),Shenzhen,China 2.Dalian Municipal Central Hospital,Dalian,China
第一作者单位	南方科技大学第一附属医院
第一作者的第一单位	南方科技大学第一附属医院
推荐引用方式 GB/T 7714	Liu，Chang,Li，Tian Yu,Chen，Yong,et al. Tendon microstructural disruption promotes tendon-derived stem cells to express chondrogenic genes by activating endoplasmic reticulum stress[J]. JOURNAL OF ORTHOPAEDIC RESEARCH,2022,41(2).
APA	Liu，Chang,Li，Tian Yu,Chen，Yong,Yang，Huan Huan,&Sun，Yu Long.(2022).Tendon microstructural disruption promotes tendon-derived stem cells to express chondrogenic genes by activating endoplasmic reticulum stress.JOURNAL OF ORTHOPAEDIC RESEARCH,41(2).
MLA	Liu，Chang,et al."Tendon microstructural disruption promotes tendon-derived stem cells to express chondrogenic genes by activating endoplasmic reticulum stress".JOURNAL OF ORTHOPAEDIC RESEARCH 41.2(2022).