Identification of Microbiome Etiology Associated With Drug Resistance in Pleural Empyema

Chen，Zhaoyan1; Cheng，Hang2; Cai，Zhao2; Wei，Qingjun3; Li，Jinlong4; Liang，Jinhua4; Zhang，Wenshu4; Yu，Zhijian5; Liu，Dongjing6; Liu，Lei6; Zhang，Zhenqiang7; Wang，Ke4; Yang，Liang2

2021-03-16

10.3389/fcimb.2021.637018

Frontiers in Cellular and Infection Microbiology   影响因子和分区

2235-2988

Identification of the offending organism and appropriate antimicrobial therapy are crucial for treating empyema. Diagnosis of empyema is largely obscured by the conventional bacterial cultivation and PCR process that has relatively low sensitivity, leading to limited understanding of the etiopathogenesis, microbiology, and role of antibiotics in the pleural cavity. To expand our understanding of its pathophysiology, we have carried out a metagenomic snapshot of the pleural effusion from 45 empyema patients by Illumina sequencing platform to assess its taxonomic, and antibiotic resistome structure. Our results showed that the variation of microbiota in the pleural effusion is generally stratified, not continuous. There are two distinct microbiome clusters observed in the forty-five samples: HA-SA type and LA-SA type. The categorization is mostly driven by species composition: HA-SA type is marked by Staphylococcus aureus as the core species, with other enriched 6 bacteria and 3 fungi, forming a low diversity and highly stable microbial community; whereas the LA-SA type has a more diverse microbial community with a distinct set of bacterial species that are assumed to be the oral origin. The microbial community does not shape the dominant antibiotic resistance classes which were common in the two types, while the increase of microbial diversity was correlated with the increase in antibiotic resistance genes. The existence of well-balanced microbial symbiotic states might respond differently to pathogen colonization and drug intake. This study provides a deeper understanding of the pathobiology of pleural empyema and suggests that potential resistance genes may hinder the antimicrobial therapy of empyema.

community structure empyema metagenomic microbiome resistome Staphylococcus aureus

SCI

英语

通讯

WOS:000634728700001

2-s2.0-85103415497

Scopus

1.Intensive Care Unit,The First Affiliated Hospital of Guangxi Medical University,Nanning,China
2.School of Medicine,Southern University of Science and Technology,Shenzhen,China
3.Department of Orthopedic Trauma and Hand Surgery,The First Affiliated Hospital of Guangxi Medical University,Nanning,China
4.Pulmonary and Critical Care Medicine,The First Affiliated Hospital of Guangxi Medical University,Nanning,China
5.Department of Infectious Diseases,Shenzhen Key Laboratory for Endogenous Infection,Shenzhen Nanshan People’s Hospital of Shenzhen University,Shenzhen,China
6.National Clinical Research Center for Infectious Diseases,Shenzhen Third People’s Hospital,Southern University of Science and Technology,Shenzhen,China
7.Department of Respiratory and Critical Care Medicine,Liuzhou People’s Hospital,Liuzhou,China

Chen，Zhaoyan,Cheng，Hang,Cai，Zhao,et al. Identification of Microbiome Etiology Associated With Drug Resistance in Pleural Empyema[J]. Frontiers in Cellular and Infection Microbiology,2021,11.

Chen，Zhaoyan.,Cheng，Hang.,Cai，Zhao.,Wei，Qingjun.,Li，Jinlong.,...&Yang，Liang.(2021).Identification of Microbiome Etiology Associated With Drug Resistance in Pleural Empyema.Frontiers in Cellular and Infection Microbiology,11.

Chen，Zhaoyan,et al."Identification of Microbiome Etiology Associated With Drug Resistance in Pleural Empyema".Frontiers in Cellular and Infection Microbiology 11(2021).