南方科技大学知识苑(SUSTech KC): 基于光声和光学相干层析技术的多模态口腔成像研究

题名	基于光声和光学相干层析技术的多模态口腔成像研究
姓名	李颖
姓名拼音	LI Ying
学号	11930398
学位类型	硕士
学位专业	071000生物学
学科门类/专业学位类别	07 理学
导师	奚磊
导师单位	生物医学工程系
论文答辩日期	2022-05-11
论文提交日期	2022-06-13
学位授予单位	南方科技大学
学位授予地点	深圳
摘要	口腔疾病具有发病率高，种类繁多等特点，我国口腔疾病发病率高达 97.6%。由于口腔功能和位置的特殊性，反复多发的口腔疾病给患者日常生活造成严重困扰。科学技术和社会经济的发展推动医疗水平不断提升，人们也逐渐意识到口腔健康的重要性。口腔疾病早发现、早治疗能够极大减轻患者的痛苦，如何快速、准确地诊断早期口腔疾病成为医疗技术发展的一大迫切需求。通过影像学结果可快速、直观、准确地判断口腔内部环境健康状态。光声成像作为一种新兴生物医学成像方法，具有非入侵、高分辨率、高对比度等特点，能够获得清晰的血管成像；光学相干层析成像也是近几十年发展起来的生物医学成像技术，其根据生物组织后向散射光携带的生物组织内部信息，可以获得生物组织高轴向分辨率的层析成像，在临床上已有成熟应用。口腔内部发生病变时，病变处毛细血管微循环和组织分层结构会发生变化。因此结合光声成像和光学相干层析成像两种成像技术，可有效探测口腔疾病早期的血管微循环变化以及组织形态结构改变，为口腔疾病早期诊断提供新的方法。而以往报道的光声-光学相干层析多模态成像系统或受限于系统体积庞大、集成度低，或受限于时间、空间分辨率低，均不适用于口腔疾病的早期诊断。为突破上述限制，本文搭建了一套小型化光声-光学相干层析多模态成像系统，利用微机电扫描振镜提高设备集成度。本文所实现的成像探头最终体积仅 45×20×15mm3，可灵活地采集口腔内不同区域的成像结果。通过仿体成像和动物实验验证系统光声横向分辨率可达 3.9μm，轴向分辨率为 120μm；光学相干层析成像横向分辨率为 5.7μm，轴向分辨率为 6.0μm。探头能获得清晰的血管网络和组织结构成像，证实此成像探头对活体生物组织成像性能良好。同时，使用该系统采集了健康志愿者口腔不同区域成像数据，观察到不同区域血管和组织结构形态差异，验证了该系统的临床应用潜力。
其他摘要	Oral diseases have the characteristics of high incidence, and great variety. The morbidity of oral disease in China is as high as 97.6%. Due to the particularity of oral function and location, repeated oral diseases cause serious distress to patients’ daily life. The development of science and economy have promoted the continuous improvement of medical level, and people become aware of prevention and treatment of oral diseases gradually. Early detection and treatment of oral diseases can greatly reduce the pain of patients. How to screen early oral diseases quickly, precisely has become an urgent need for the development of oral medical technology. Through imaging results can quickly, visually and accurately judge the health status of the internal oral cavity. As an emerging biomedical imaging method, photoacoustic imaging has the characteristics of non-invasive, high resolution, and high contrast, which can obtain clear imaging of vascular network. Optical coherence tomography is also a biomedical imaging technology developed in recent decades and has been applied in clinical practice. According to the information carried by the backscattered light of biological tissues, it can get the tomography result with high axial resolution. When disease occurs in the oral cavity, the capillary microcirculation and tissue stratification structure at the lesions will change. Therefore, the combination of photoacoustic imaging and optical coherence tomography can effectively detect the changes of vascular microcirculation and tissue morphological structure in the early stage of oral diseases. It will provide a new method for the early diagnosis of oral diseases. However, the previously reported photoacoustic-optical coherence tomography multi-modal systems are not suitable for the early diagnosis of oral diseases due to the bulky system volume, low integration, temporal and spatial resolution. In order to break through the limitations mentioned above, a hand-held miniaturized multi-modal system of photoacoustic and optical coherence tomography is proposed and implemented in this thesis. Using micro-electro- mechanical scanning mirror make the imaging probe more integrated. The volume of the imaging probe in this thesis is only 45×20×15mm3, which can flexibly collect the imaging results of various areas in the oral cavity. Through the phantom and animal experiments, it verifyied the photoacoustic lateral resolution can reach 3.9μm and the axial resolution is 120μm; The lateral resolution of optical coherence tomography is 5.7μm, and the axial resolution is 6.0μm. It can obtain clear capillary network and tissue layered structure images of mice, which confirmed the system has good imaging ability for in vivo biological tissue. Meanwhile, we used this multi-modality system to collect the imaging results of the different oral areas of a healthy volunteer, and observed the differences in the structure and morphology of blood vessels and tissues in different regions. It verified the potential of clinical application of the system.
关键词	口腔疾病光声成像光学相干层析成像
其他关键词	Oral Disease Photoacoustic Optical Coherence Tomography
语种	中文
培养类别	独立培养
入学年份	2019
学位授予年份	2022-06
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所在学位评定分委会	生物系
国内图书分类号	TP391.41
来源库	人工提交
成果类型	学位论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/335697
专题	工学院_生物医学工程系
推荐引用方式 GB/T 7714	李颖. 基于光声和光学相干层析技术的多模态口腔成像研究[D]. 深圳. 南方科技大学,2022.

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