A molecular framework of ethylene-mediated fruit growth and ripening processes in tomato

Huang, Wei1,2; Hu, Nan3; Xiao, Zhina1,2; Qiu, Yuping1,2; Yang, Yan1,2,4; Yang, Jie1,2,4; Mao, Xin1,2,4; Wang, Yichuan1,2; Li, Zhengguo3; Guo, Hongwei1,2

2022-05-01

10.1093/plcell/koac146

PLANT CELL   影响因子和分区

1040-4651

1532-298X

["Although the role of ethylene in tomato (Solanum lycopersicum) fruit ripening has been intensively studied, its role in tomato fruit growth remains poorly understood. In addition, the relationship between ethylene and the developmental factors NON-RIPENING (NOR) and RIPENING INHIBITOR (RIN) during ripening is under debate. Here, we carried out comprehensive genetic analyses of genome-edited mutants of tomato ETHYLENE INSENSITIVE 2 (SlEIN2), four EIN3-like genes (SlEIL1-4), and three EIN3 BINDING F-box protein genes (SlEBF1-3). Both slein2-1 and the high-order sleil mutant (sleil1 sleil2 sleil3/SlEIL3 sleil4) showed reduced fruit size, mainly due to decreased auxin biosynthesis. During fruit maturation, slein2 mutants displayed the complete cessation of ripening, which was partially rescued by slebf1 but not slebf2 or slebf3. We also discovered that ethylene directly activates the expression of the developmental genes NOR, RIN, and FRUITFULL1 (FUL1) via SlEIL proteins. Indeed, overexpressing these genes partially rescued the ripening defects of slein2-1. Finally, the signal intensity of the ethylene burst during fruit maturation was intimately connected with the progression of full ripeness. Collectively, our work uncovers a critical role of ethylene in fruit growth and supports a molecular framework of ripening control in which the developmental factors NOR, RIN, and FUL1 act downstream of ethylene signaling.","Low-level ethylene production promotes tomato fruit growth by modulating auxin biosynthesis/signaling, and the ethylene burst induces fruit ripening by activating fruit development-related genes."]

SCI

英语

ESI高被引 ; NI论文

第一 ; 通讯

Shenzhen Science and Technology Program[KQTD20190929173906742] ; National Key Research and Development Program of China[2019YFA0903904] ; Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes (SUSTech)[2019KSYS006] ; National Nature Science Foundation of China[32070322,"K20227505"]

Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology

Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology

WOS:000805342500001

OXFORD UNIV PRESS INC

PLANT & ANIMAL SCIENCE

Web of Science

1.Southern Univ Sci & Technol SUSTech, Inst Plant & Food Sci, Dept Biol, Shenzhen 518055, Peoples R China
2.Southern Univ Sci & Technol SUSTech, Guangdong Higher Educ Inst, Key Lab Mol Design Plant Cell Factory, Shenzhen 518055, Peoples R China
3.Chongqing Univ, Sch Life Sci, Key Lab Plant Hormones & Dev Regulat Chongqing, Chongqing 401331, Peoples R China
4.Southern Univ Sci & Technol SUSTech, Acad Adv Interdisciplinary Studies, Shenzhen 518055, Peoples R China

Huang, Wei,Hu, Nan,Xiao, Zhina,et al. A molecular framework of ethylene-mediated fruit growth and ripening processes in tomato[J]. PLANT CELL,2022.

Huang, Wei.,Hu, Nan.,Xiao, Zhina.,Qiu, Yuping.,Yang, Yan.,...&Guo, Hongwei.(2022).A molecular framework of ethylene-mediated fruit growth and ripening processes in tomato.PLANT CELL.

Huang, Wei,et al."A molecular framework of ethylene-mediated fruit growth and ripening processes in tomato".PLANT CELL (2022).