南方科技大学知识苑(SUSTech KC): FAM83A antisense RNA 1 (FAM83A-AS1) silencing impairs cell proliferation and induces autophagy via MET-AMPKalpha signaling in lung adenocarcinoma

题名	FAM83A antisense RNA 1 (FAM83A-AS1) silencing impairs cell proliferation and induces autophagy via MET-AMPKalpha signaling in lung adenocarcinoma
作者	Zhao, Huijie1 ; Wang, Yinghan 2; Wu, Xing 1; Zeng, Xaofei 1; Lin, Baoyue 1; Hu, Shengmin1 ; Zhang, Shenglin1 ; Li, Yu1 ; Zhou, Zhiqing1 ; Zhou, Yujie1 ; Du, Changzheng 1; Beer, David G.3; Bai, Shengbin 4; Chen, Guoan1
通讯作者	Chen, Guoan
发表日期	2022-05-02
DOI	10.1080/21655979.2022.2081457
发表期刊	Bioengineered 影响因子和分区
ISSN	2165-5979
EISSN	2165-5987
卷号	13 期号:5
摘要	Studies demonstrate that long non-coding RNAs (lncRNAs) play vital roles in cancer progression. However, the expression pattern and molecular mechanisms of lncRNA FAM83A-AS1 in lung cancer remain largely unclear. Here, we analyzed FAM83A-AS1 expression in lung cancer tissues from three RNA-sequencing (RNA-Seq) datasets and validated these results using quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) in an independent set of lung adenocarcinoma. Cell proliferation, migration, invasion, and autophagy were analyzed after knockdown FAM83A-AS1 with siRNAs. The underlying molecular mechanisms of FAM83A-AS1 were performed by Western blot, qRT-PCR, and RNA-seq analysis. We found that FAM83A-AS1 was up-regulated in lung cancer and elevated expression was associated with poor patient survival. These results were confirmed using RT-PCR in an independent set of lung cancer. Functional study indicated that FAM83A-AS1 knockdown reduced cell proliferation, migration, invasion, and colony formation in cancer cells. FAM83A-AS1 silencing induced autophagy and cell cycle arrest at G2. Mechanistically, serval oncogenic proteins such as EGFR, MET, PI3K, and K-RAS were decreased upon FAM83A-AS1 silencing, while phosphor AMPK alpha and ULK1 were increased. Based on the above results, we believe that FAM83A-AS1 may have potential as a diagnosis/prognosis marker and its oncogenic role and autophagy regulation may be through MET-AMPK alpha signaling, which could lead to potential targeting for lung cancer therapy.
关键词	Lung adenocarcinoma lncRNA FAM83A-AS1 MET autophagy
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	第一 ; 通讯
资助项目	Shenzhen Municipal Science and Technology Innovation Commission Foundation[2021-255] ; National Natural Science Foundation of China (NSFC)[32070625]
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000804154400001
出版者	TAYLOR & FRANCIS INC
来源库	Web of Science
引用统计	被引频次[WOS]：11
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/335797
专题	南方科技大学医学院生命科学学院南方科技大学医学院_人类细胞生物和遗传学系
作者单位	1.Southern Univ Sci & Technol, Sch Med, Shenzhen, Guangdong, Peoples R China 2.Southern Univ Sci & Technol, Sch Life Sci, Shenzhen, Guangdong, Peoples R China 3.Univ Michigan, Dept Surg, Med Sch, Ann Arbor, MI 48109 USA 4.Xinjiang Med Univ, Basic Med Coll, Dept Histol & Embryol, Urumqi, Peoples R China
第一作者单位	南方科技大学医学院
通讯作者单位	南方科技大学医学院
第一作者的第一单位	南方科技大学医学院
推荐引用方式 GB/T 7714	Zhao, Huijie,Wang, Yinghan,Wu, Xing,et al. FAM83A antisense RNA 1 (FAM83A-AS1) silencing impairs cell proliferation and induces autophagy via MET-AMPKalpha signaling in lung adenocarcinoma[J]. Bioengineered,2022,13(5).
APA	Zhao, Huijie.,Wang, Yinghan.,Wu, Xing.,Zeng, Xaofei.,Lin, Baoyue.,...&Chen, Guoan.(2022).FAM83A antisense RNA 1 (FAM83A-AS1) silencing impairs cell proliferation and induces autophagy via MET-AMPKalpha signaling in lung adenocarcinoma.Bioengineered,13(5).
MLA	Zhao, Huijie,et al."FAM83A antisense RNA 1 (FAM83A-AS1) silencing impairs cell proliferation and induces autophagy via MET-AMPKalpha signaling in lung adenocarcinoma".Bioengineered 13.5(2022).