Metabolic control of arginine and ornithine levels paces the progression of leaf senescence

Liebsch, Daniela1,7; Juvany, Marta1; Li, Zhonghai2; Wang, Hou-Ling2; Ziolkowska, Agnieszka1; Chrobok, Daria1; Boussardon, Clement1; Wen, Xing3; Law, Simon R.1; Janeckova, Helena4; Brouwer, Bastiaan1,8; Linden, Pernilla1; Delhomme, Nicolas1,5; Stenlund, Hans1,5; Moritz, Thomas5,6; Gardestrom, Per1; Guo, Hongwei3; Keech, Olivier1

2022-05-01

10.1093/plphys/kiac244

PLANT PHYSIOLOGY   影响因子和分区

0032-0889

1532-2548

["Pools of arginine and ornithine generated during protein degradation can pace the progression of leaf senescence by affecting the TCA cycle, polyamine biosynthesis and the ethylene signaling pathway.","Leaf senescence can be induced by stress or aging, sometimes in a synergistic manner. It is generally acknowledged that the ability to withstand senescence-inducing conditions can provide plants with stress resilience. Although the signaling and transcriptional networks responsible for a delayed senescence phenotype, often referred to as a functional stay-green trait, have been actively investigated, very little is known about the subsequent metabolic adjustments conferring this aptitude to survival. First, using the individually darkened leaf (IDL) experimental setup, we compared IDLs of wild-type (WT) Arabidopsis (Arabidopsis thaliana) to several stay-green contexts, that is IDLs of two functional stay-green mutant lines, oresara1-2 (ore1-2) and an allele of phytochrome-interacting factor 5 (pif5), as well as to leaves from a WT plant entirely darkened (DP). We provide compelling evidence that arginine and ornithine, which accumulate in all stay-green contexts-likely due to the lack of induction of amino acids (AAs) transport-can delay the progression of senescence by fueling the Krebs cycle or the production of polyamines (PAs). Secondly, we show that the conversion of putrescine to spermidine (SPD) is controlled in an age-dependent manner. Thirdly, we demonstrate that SPD represses senescence via interference with ethylene signaling by stabilizing the ETHYLENE BINDING FACTOR1 and 2 (EBF1/2) complex. Taken together, our results identify arginine and ornithine as central metabolites influencing the stress- and age-dependent progression of leaf senescence. We propose that the regulatory loop between the pace of the AA export and the progression of leaf senescence provides the plant with a mechanism to fine-tune the induction of cell death in leaves, which, if triggered unnecessarily, can impede nutrient remobilization and thus plant growth and survival."]

SCI

英语

通讯

Swedish research council "VetenskapsRadet"[621-2014-4688] ; Carl Tryggers Stiftelse["CTS14-247","CTS15-262"] ; National Natural Science Foundation of China[31970196,31570286,31900173] ; Shenzhen Science and Technology Program[KQTD20190929173906742] ; National Key Research and Development Program of China[2019YFA0903904] ; Knut and Alice Wallenberg Foundation["KAW 2016.0341",2016.0352] ; Swedish Governmental Agency for Innovation Systems (VINNOVA)[2016-00504]

Plant Sciences

Plant Sciences

WOS:000803838800001

OXFORD UNIV PRESS INC

PLANT & ANIMAL SCIENCE

Web of Science

1.Umea Univ, Dept Plant Physiol, Umea Plant Sci Ctr, S-90187 Umea, Sweden
2.Beijing Forestry Univ, Coll Biol Sci & Technol, Natl Engn Lab Tree Breeding, Beijing 100083, Peoples R China
3.Southern Univ Sci & Technol SUSTech, Inst Plant & Food Sci, Dept Biol, Shenzhen 518055, Guangdong, Peoples R China
4.Palacky Univ, Dept Biophys, Fac Sci, Ctr Reg Hana Biotechnol & Agr Res, Olomouc 78371, Czech Republic
5.Swedish Univ Agr Sci, Umea Plant Sci Ctr, Dept Forest Genet & Plant Physiol, S-90183 Umea, Sweden
6.Univ Copenhagen, Novo Nordisk Ctr Basic Metab Res, DK-2200 Copenhagen N, Denmark
7.IBR CONICET, Inst Biol Mol & Celular Rosario, Ocampo & Esmeralda S-N, RA-2000 Rosario, Argentina
8.Wageningen Food & Biobased Res, Wageningen Campus,POB 17, NL-6700 AA Wageningen, Netherlands

Liebsch, Daniela,Juvany, Marta,Li, Zhonghai,et al. Metabolic control of arginine and ornithine levels paces the progression of leaf senescence[J]. PLANT PHYSIOLOGY,2022.

Liebsch, Daniela.,Juvany, Marta.,Li, Zhonghai.,Wang, Hou-Ling.,Ziolkowska, Agnieszka.,...&Keech, Olivier.(2022).Metabolic control of arginine and ornithine levels paces the progression of leaf senescence.PLANT PHYSIOLOGY.

Liebsch, Daniela,et al."Metabolic control of arginine and ornithine levels paces the progression of leaf senescence".PLANT PHYSIOLOGY (2022).