南方科技大学知识苑(SUSTech KC): Identification and application of a pair of noncompeting monoclonal antibodies broadly binding to the nucleocapsid proteins of SARS-CoV-2 variants including Omicron

题名	Identification and application of a pair of noncompeting monoclonal antibodies broadly binding to the nucleocapsid proteins of SARS-CoV-2 variants including Omicron
作者	Zhou, Bing1 ; Cheng, Lin 1; Song, Shuo1 ; Guo, Huimin 1; Shen, Senlin1 ; Wang, Haiyan 1; Ge, Xiangyang 1; Liu, Lei1 ; Ju, Bin 1,2; Zhang, Zheng 1,2,3
通讯作者	Liu, Lei; Ju, Bin; Zhang, Zheng
发表日期	2022-05-28
DOI	10.1186/s12985-022-01827-w
发表期刊	VIROLOGY JOURNAL 影响因子和分区
EISSN	1743-422X
卷号	19 期号:1
摘要	The SARS-CoV-2 nucleocapsid protein (NP) is an important indicator for the virus infection, highlighting the crucial role of NP-specific monoclonal antibodies (mAbs) used in multiple biochemical assays and clinical diagnosis for detecting the NP antigen. Here, we reported a pair of noncompeting human NP-specific mAbs, named P301-F7 and P301-H5, targeting two distinct linear epitopes on SARS-CoV-2 or SARS-CoV. We evaluated the application of P301-F7 in the analysis of enzyme linked immunosorbent assay, western blot, flow cytometry, immunofluorescence, and focus reduction neutralization test. We for the first time report a broad mAb effectively recognizing various live viruses of SARS-CoV-2 variants including Alpha, Beta, Delta, and Omicron, indicating a wide range of application prospects.
关键词	SARS-CoV-2 variants Delta Omicron Nucleocapsid protein Monoclonal antibody
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	第一 ; 通讯
资助项目	National Science Fund for Distinguished Young Scholars[82025022] ; National Natural Science Foundation of China[92169204,82002140,82171752,82101861] ; National Key Plan for Scientific Research and Development of China[2021YFC0864500] ; Guangdong Basic and Applied Basic Research Foundation["2021B1515020034","2021A1515011009","2020A1515110656","2019A1515011197"] ; Shenzhen Science and Technology Program[RCYX20200714114700046] ; Science and Technology Innovation Committee of Shenzhen Municipality["JSGG20200207155251653","JSGG20200807171401008","JSGG20210901145200002","KQTD20200909113758004"] ; Shenzhen Natural Science Foundation["JCYJ20210324131606018","JCYJ20190809115617365"]
WOS研究方向	Virology
WOS类目	Virology
WOS记录号	WOS:000801166000001
出版者	BMC
Scopus记录号	2-s2.0-85130823381
来源库	Web of Science
引用统计	被引频次[WOS]：10
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/335820
专题	南方科技大学医学院南方科技大学第二附属医院
作者单位	1.Southern Univ Sci & Technol, Shenzhen Third Peoples Hosp, Inst Hepatol, Natl Clin Res Ctr Infect Dis,Sch Med, Shenzhen 518112, Guangdong, Peoples R China 2.Guangdong Key Lab Antiinfect Drug Qual Evaluat, Shenzhen 518112, Guangdong, Peoples R China 3.Chinese Acad Med Sci, Shenzhen Res Ctr Communicable Dis Diag & Treatmen, Shenzhen 518112, Guangdong, Peoples R China
第一作者单位	南方科技大学医学院; 南方科技大学第二附属医院
通讯作者单位	南方科技大学医学院; 南方科技大学第二附属医院
第一作者的第一单位	南方科技大学医学院; 南方科技大学第二附属医院
推荐引用方式 GB/T 7714	Zhou, Bing,Cheng, Lin,Song, Shuo,et al. Identification and application of a pair of noncompeting monoclonal antibodies broadly binding to the nucleocapsid proteins of SARS-CoV-2 variants including Omicron[J]. VIROLOGY JOURNAL,2022,19(1).
APA	Zhou, Bing.,Cheng, Lin.,Song, Shuo.,Guo, Huimin.,Shen, Senlin.,...&Zhang, Zheng.(2022).Identification and application of a pair of noncompeting monoclonal antibodies broadly binding to the nucleocapsid proteins of SARS-CoV-2 variants including Omicron.VIROLOGY JOURNAL,19(1).
MLA	Zhou, Bing,et al."Identification and application of a pair of noncompeting monoclonal antibodies broadly binding to the nucleocapsid proteins of SARS-CoV-2 variants including Omicron".VIROLOGY JOURNAL 19.1(2022).