Vibrio cholerae senses human enteric α-defensin 5 through a CarSR two-component system to promote bacterial pathogenicity

Liu，Yutao1; Xu，Tingting2; Wang，Qian1; Huang，Junxi2; Zhu，Yangfei3; Liu，Xingmei1; Liu，Ruiying1; Yang，Bin1; Zhou，Kai2

2022-12-01

10.1038/s42003-022-03525-3

Communications Biology   影响因子和分区

2399-3642

Vibrio cholerae (V. cholerae) is an aquatic bacterium responsible for acute and fatal cholera outbreaks worldwide. When V. cholerae is ingested, the bacteria colonize the epithelium of the small intestine and stimulate the Paneth cells to produce large amounts of cationic antimicrobial peptides (CAMPs). Human defensin 5 (HD-5) is the most abundant CAMPs in the small intestine. However, the role of the V. cholerae response to HD-5 remains unclear. Here we show that HD-5 significantly upregulates virulence gene expression. Moreover, a two-component system, CarSR (or RstAB), is essential for V. cholerae virulence gene expression in the presence of HD-5. Finally, phosphorylated CarR can directly bind to the promoter region of TcpP, activating transcription of tcpP, which in turn activates downstream virulence genes to promote V. cholerae colonization. In conclusion, this study reveals a virulence-regulating pathway, in which the CarSR two-component regulatory system senses HD-5 to activate virulence genes expression in V. cholerae.

SCI

英语

通讯

National Outstanding Youth Science Fund Project of National Natural Science Foundation of China[32100144];National Outstanding Youth Science Fund Project of National Natural Science Foundation of China[81902030];National Outstanding Youth Science Fund Project of National Natural Science Foundation of China[82172330];

Life Sciences & Biomedicine - Other Topics ; Science & Technology - Other Topics

Biology ; Multidisciplinary Sciences

WOS:000808430700003

NATURE PORTFOLIO

2-s2.0-85131656655

Scopus

1.TEDA Institute of Biological Sciences and Biotechnology,TEDA,Nankai University,Tianjin,China
2.The Second Clinical Medical College,Jinan University,The First Affiliated Hospital,Southern University of Science and Technology,Shenzhen People’s Hospital,Shenzhen Institute of Respiratory Diseases,Shenzhen,Guangdong,China
3.The Second Clinical Medical College,Jinan University,The First Affiliated Hospital,Southern University of Science and Technology,Laboratory Department,Shenzhen People’s Hospital,Shenzhen,Guangdong,China

Liu，Yutao,Xu，Tingting,Wang，Qian,et al. Vibrio cholerae senses human enteric α-defensin 5 through a CarSR two-component system to promote bacterial pathogenicity[J]. Communications Biology,2022,5(1).

Liu，Yutao.,Xu，Tingting.,Wang，Qian.,Huang，Junxi.,Zhu，Yangfei.,...&Zhou，Kai.(2022).Vibrio cholerae senses human enteric α-defensin 5 through a CarSR two-component system to promote bacterial pathogenicity.Communications Biology,5(1).

Liu，Yutao,et al."Vibrio cholerae senses human enteric α-defensin 5 through a CarSR two-component system to promote bacterial pathogenicity".Communications Biology 5.1(2022).