南方科技大学知识苑(SUSTech KC): Lockd promotes myoblast proliferation and muscle regeneration via binding with DHX36 to facilitate 5' UTR rG4 unwinding and Anp32e translation

题名	Lockd promotes myoblast proliferation and muscle regeneration via binding with DHX36 to facilitate 5' UTR rG4 unwinding and Anp32e translation
作者	Chen，Xiaona 1; Xue，Guang 1; Zhao，Jieyu 2; Zhang，Yuwei 3; Zhang，Suyang 4; Wang，Wen5 ; Li，Yang 4; Yuan，Jie 3; He，Liangqiang 6; Chan，Chun Yin 2; Liu，Yan 7; Chen，Wei5 ; Zhao，Yu 1; Hu，Ping 7; Sun，Hao 3; Kwok，Chun Kit 8; Wang，Huating 4
通讯作者	Sun，Hao; Kwok，Chun Kit; Wang，Huating
共同第一作者	Xue，Guang
发表日期	2022-06-07
DOI	10.1016/j.celrep.2022.110927
发表期刊	Cell Reports 影响因子和分区
ISSN	2211-1247
EISSN	2211-1247
卷号	39 期号:10
摘要	Adult muscle stem cells, also known as satellite cells (SCs), play pivotal roles in muscle regeneration, and long non-coding RNA (lncRNA) functions in SCs remain largely unknown. Here, we identify a lncRNA, Lockd, which is induced in activated SCs upon acute muscle injury. We demonstrate that Lockd promotes SC proliferation; deletion of Lockd leads to cell-cycle arrest, and in vivo repression of Lockd in mouse muscles hinders regeneration process. Mechanistically, we show that Lockd directly interacts with RNA helicase DHX36 and the 5'end of Lockd possesses the strongest binding with DHX36. Furthermore, we demonstrate that Lockd stabilizes the interaction between DHX36 and EIF3B proteins; synergistically, this complex unwinds the RNA G-quadruplex (rG4) structure formed at Anp32e mRNA 5' UTR and promotes the translation of ANP32E protein, which is required for myoblast proliferation. Altogether, our findings identify a regulatory Lockd/DHX36/Anp32e axis that promotes myoblast proliferation and acute-injury-induced muscle regeneration.
关键词	ANP32E CP: Molecular Biology CP: Stem Cell Research DHX36 EIF3B Lockd Muscle Regeneration Post-transcriptional Regulation RNA G-quadruplexes Satellite Cells Translational Regulation
相关链接	[Scopus记录]
收录类别	SCI
语种	英语
学校署名	其他
资助项目	National Natural Science Foundation of China (NSFC)[
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000835660300006
出版者	CELL PRESS
Scopus记录号	2-s2.0-85131626286
来源库	Scopus
引用统计	被引频次[WOS]：6
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/336241
专题	生命科学学院_生物系生命科学学院
作者单位	1.Department of Orthopaedics and Traumatology,Li Ka Shing Institute of Health Sciences,Chinese University of Hong Kong,Hong Kong SAR,China 2.Department of Chemistry and State Key Laboratory of Marine Pollution,City University of Hong Kong,Hong Kong SAR,Kowloon Tong,China 3.Department of Chemical Pathology,Li Ka Shing Institute of Health Sciences,Chinese University of Hong Kong,Hong Kong SAR,China 4.Department of Orthopaedics and Traumatology, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China; Center for Neuromusculoskeletal Restorative Medicine, Hong Kong Science Park, New Territories, Hong Kong SAR, China 5.Department of Biology,Southern University of Science and Technology,Shenzhen,China 6.Department of Chemical Pathology, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China; Center for Neuromusculoskeletal Restorative Medicine, Hong Kong Science Park, New Territories, Hong Kong SAR, China 7.Guangzhou Laboratory,Guanghzou International Bio lsland,Guangzhou,No. 9 XingDaoHuan Road,510005,China 8.Department of Chemistry and State Key Laboratory of Marine Pollution, City University of Hong Kong, Kowloon Tong, Hong Kong SAR, China; Shenzhen Research Institute of City University of Hong Kong, Shenzhen, China
推荐引用方式 GB/T 7714	Chen，Xiaona,Xue，Guang,Zhao，Jieyu,et al. Lockd promotes myoblast proliferation and muscle regeneration via binding with DHX36 to facilitate 5' UTR rG4 unwinding and Anp32e translation[J]. Cell Reports,2022,39(10).
APA	Chen，Xiaona.,Xue，Guang.,Zhao，Jieyu.,Zhang，Yuwei.,Zhang，Suyang.,...&Wang，Huating.(2022).Lockd promotes myoblast proliferation and muscle regeneration via binding with DHX36 to facilitate 5' UTR rG4 unwinding and Anp32e translation.Cell Reports,39(10).
MLA	Chen，Xiaona,et al."Lockd promotes myoblast proliferation and muscle regeneration via binding with DHX36 to facilitate 5' UTR rG4 unwinding and Anp32e translation".Cell Reports 39.10(2022).