Artificial Dermal Scaffold Loaded with Platelet-Rich Plasma Promotes Wound Healing in Pigs by Favoring Angiogenesis

Li，Zhi Hao1,2; Wu，Guo Feng3; Song，Hao Qiang1; Huang，Kui4; Wu，Bo4; Xu，Xiao Long2; Zhu，Li Xin1

2022-06-06

10.12659/MSM.936186

MEDICAL SCIENCE MONITOR   影响因子和分区

1234-1010

1643-3750

Background: The development of artificial dermis provides a new therapeutic method for full-thickness skin defects. However, the slow regeneration of blood vessels in the wound site still cannot be solved perfectly. In our study, we combined platelet-rich plasma (PRP) with Lando artificial dermal scaffold to promote vascular regeneration and wound healing in pigs. Material/Methods: First, PRP was compounded with the artificial dermal scaffold. Then, this material was co-cultured with human vascular endothelial cells (HUVECs) and the growth and proliferation of HUVECs were assessed. Bama miniature pigs wound models were fabricated, the materials were transplanted into the skin defect, and wound healing and blood vessel regeneration were assessed by HE staining and CD31 immunohistochemistry. Results: Scanning electron microscopy (SEM) showed that PRP formed round particles on the surface of the artificial dermis material. Cell co-culture experiments showed that the PRP composite artificial dermal scaffold can promote the growth and proliferation of HUVECs. CCK8 experiments demonstrated that the number of cells in the PRP composites group on days 2, 3, 4, and 5 was higher than that in the material alone group (P<0.01). The results of animal experiments showed that PRP composite artificial dermal material can promote wound healing. Histological staining and immunohistochemical staining indicated that the PRP composites group promoted epithelial tissue thickening and blood vessel regeneration in wounds (P<0.001). Conclusions: Our experimental results showed that the artificial dermal scaffold loaded with platelet-rich plasma can promote the revascularization of wounds and accelerated wound healing.

Angiogenesis Inducing Agents Pacemaker, Artificial Platelet-Rich Plasma Skin, Artificial Wound Healing

SCI

英语

其他

Natural Science Foundation of Hubei Province[2020CFB687]

Research & Experimental Medicine

Medicine, Research & Experimental

WOS:000809411300001

INT SCIENTIFIC INFORMATION, INC

CLINICAL MEDICINE

2-s2.0-85131693863

Scopus

1.Department of Orthopedics,Zhujiang Hospital,Southern Medical University,Guangzhou,Guangdong,China
2.Department of Orthopedics,Jingzhou Central Hospital,Jingzhou,Hubei,China
3.Department of Orthopedics,The Third Affiliated Hospital of South University of Science and Technology,Shenzhen,Guangdong,China
4.Department of Orthopedics,The First Hospital of Yangtze University,Jingzhou,Hubei,China

Li，Zhi Hao,Wu，Guo Feng,Song，Hao Qiang,et al. Artificial Dermal Scaffold Loaded with Platelet-Rich Plasma Promotes Wound Healing in Pigs by Favoring Angiogenesis[J]. MEDICAL SCIENCE MONITOR,2022,28.

Li，Zhi Hao.,Wu，Guo Feng.,Song，Hao Qiang.,Huang，Kui.,Wu，Bo.,...&Zhu，Li Xin.(2022).Artificial Dermal Scaffold Loaded with Platelet-Rich Plasma Promotes Wound Healing in Pigs by Favoring Angiogenesis.MEDICAL SCIENCE MONITOR,28.

Li，Zhi Hao,et al."Artificial Dermal Scaffold Loaded with Platelet-Rich Plasma Promotes Wound Healing in Pigs by Favoring Angiogenesis".MEDICAL SCIENCE MONITOR 28(2022).