南方科技大学知识苑(SUSTech KC): High-resolution 3D spatiotemporal transcriptomic maps of developing Drosophila embryos and larvae

题名	High-resolution 3D spatiotemporal transcriptomic maps of developing Drosophila embryos and larvae
作者	Wang，Mingyue1 ; Hu，Qinan2 ; Lv，Tianhang 3; Wang，Yuhang 4; Lan，Qing 5; Xiang，Rong 5; Tu，Zhencheng 3; Wei，Yanrong 6; Han，Kai 7; Shi，Chang 5; Guo，Junfu 5; Liu，Chao 5; Yang，Tao 8; Du，Wensi 8; An，Yanru 5; Cheng，Mengnan 3; Xu，Jiangshan 3; Lu，Haorong 9; Li，Wangsheng 8; Zhang，Shaofang 8; Chen，Ao 5; Chen，Wei10 ; Li，Yuxiang 5; Wang，Xiaoshan 5; Xu，Xun 5; Hu，Yuhui2 ; Liu，Longqi 11
通讯作者	Li，Yuxiang; Wang，Xiaoshan; Xu，Xun; Hu，Yuhui; Liu，Longqi
共同第一作者	Hu，Qinan; Lv，Tianhang; Wang，Yuhang; Lan，Qing; Xiang，Rong; Tu，Zhencheng
发表日期	2022-05-23
DOI	10.1016/j.devcel.2022.04.006
发表期刊	DEVELOPMENTAL CELL 影响因子和分区
ISSN	1534-5807
EISSN	1878-1551
卷号	57 期号:10页码:1271-1283
摘要	Drosophila has long been a successful model organism in multiple biomedical fields. Spatial gene expression patterns are critical for the understanding of complex pathways and interactions, whereas temporal gene expression changes are vital for studying highly dynamic physiological activities. Systematic studies in Drosophila are still impeded by the lack of spatiotemporal transcriptomic information. Here, utilizing spatial enhanced resolution omics-sequencing (Stereo-seq), we dissected the spatiotemporal transcriptomic changes of developing Drosophila with high resolution and sensitivity. We demonstrated that Stereo-seq data can be used for the 3D reconstruction of the spatial transcriptomes of Drosophila embryos and larvae. With these 3D models, we identified functional subregions in embryonic and larval midguts, uncovered spatial cell state dynamics of larval testis, and revealed known and potential regulons of transcription factors within their topographic background. Our data provide the Drosophila research community with useful resources of organism-wide spatiotemporally resolved transcriptomic information across developmental stages.
关键词	Drosophila Development Gene Regulatory Networks Spatial Transcriptomics Stereo-seq
相关链接	[Scopus记录]
收录类别	SCI
语种	英语
重要成果	NI论文
学校署名	第一 ; 共同第一 ; 通讯
资助项目	Shenzhen Key Laboratory of Gene Regulation and Systems Biology (China)[ZDSYS20200811144002008] ; Shenzhen Science and Technology Program (China)[KQTD20180411143432337] ; Shenzhen Key Laboratory of Single-Cell Omics (China)[ZDSYS20190902093613831] ; National Natural Science Foundation of China[32100684,81773881] ; Guangdong Provincial Key Laboratory of Genome Read and Write (China)[2017B030301011]
WOS研究方向	Cell Biology ; Developmental Biology
WOS类目	Cell Biology ; Developmental Biology
WOS记录号	WOS:000808960100006
出版者	CELL PRESS
ESI学科分类	MOLECULAR BIOLOGY & GENETICS
Scopus记录号	2-s2.0-85130556157
来源库	Scopus
引用统计	被引频次[WOS]：50
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/336257
专题	生命科学学院_生物系生命科学学院南方科技大学医学院南方科技大学医学院_药理学系
作者单位	1.BGI-Shenzhen, Shenzhen 518083, China; Shenzhen Key Laboratory of Gene Regulation and Systems Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China; Department of Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China 2.Shenzhen Key Laboratory of Gene Regulation and Systems Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China; Department of Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China; Department of Pharmacology, School of Medicine, Southern University of Science and Technology, Shenzhen 518005, China 3.BGI-Shenzhen, Shenzhen 518083, China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China 4.BGI-Shenzhen, Shenzhen 518083, China; School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China 5.BGI-Shenzhen,Shenzhen,518083,China 6.BGI College & Henan Institute of Medical and Pharmaceutical Sciences,Zhengzhou University,Zhengzhou,450000,China 7.BGI-Shenzhen,Qingdao,266555,China 8.BGI-Shenzhen,Shenzhen,China National Gene Bank,518120,China 9.China National Gene Bank, BGI-Shenzhen, Shenzhen 518120, China; Guangdong Provincial Key Laboratory of Genome Read and Write, BGI-Shenzhen, Shenzhen 518120, China 10.Shenzhen Key Laboratory of Gene Regulation and Systems Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China; Department of Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China 11.BGI-Shenzhen, Shenzhen 518083, China; Shenzhen Key Laboratory of Single-Cell Omics, Shenzhen 518083, China
第一作者单位	生物系; 生命科学学院
通讯作者单位	药理学系; 生物系; 南方科技大学医学院; 生命科学学院
第一作者的第一单位	生物系; 生命科学学院
推荐引用方式 GB/T 7714	Wang，Mingyue,Hu，Qinan,Lv，Tianhang,et al. High-resolution 3D spatiotemporal transcriptomic maps of developing Drosophila embryos and larvae[J]. DEVELOPMENTAL CELL,2022,57(10):1271-1283.
APA	Wang，Mingyue.,Hu，Qinan.,Lv，Tianhang.,Wang，Yuhang.,Lan，Qing.,...&Liu，Longqi.(2022).High-resolution 3D spatiotemporal transcriptomic maps of developing Drosophila embryos and larvae.DEVELOPMENTAL CELL,57(10),1271-1283.
MLA	Wang，Mingyue,et al."High-resolution 3D spatiotemporal transcriptomic maps of developing Drosophila embryos and larvae".DEVELOPMENTAL CELL 57.10(2022):1271-1283.