Interleukin-33 and thymic stromal lymphopoietin are primary cytokines involved in the Th1/Th2 inflammatory response in chronic secretory otitis media

Liao，Zhifang1; Tu，Bo2; Sun，Liang3; Dong，Chang3; Jiang，Hongyan3; Hu，Genwen4

2022

10.1177/1721727X221094158

European Journal of Inflammation   影响因子和分区

1721-727X

2058-7392

Background: T-helper (Th)1/Th2 inflammatory responses are responsible for secretory otitis media (SOM) development. However, the mechanisms underlying these immune responses remain unknown. This study aims to identify the primary cytokines that play essential roles in chronic SOM. Methods: Two groups were established for the present study: chronic SOM group (n = 21) and control group (n = 10). The middle ear effusion and serum samples of the expression cytokines (interleukin IL-2, IL-4, IL-5, IL-13, IL-17, IL-25, IL-33, interferon [IFN]-γ, thymic stromal lymphopoietin [TSLP], immunoglobulin IgE, and pepsins) were analyzed by enzyme-linked immunosorbent assay. Results: The levels of IL-4, IL-5, IL-13, IL-17, IL-25, IFN-γ, TLSP, pepsins, IL-2, and IL-33 (all, p < 0.001) were higher in middle ear effusion, when compared to those in serum, in chronic SOM group (non-paired sample). However, there was no significant difference in serum expression for those cytokines compared chronic SOM group and control group. The paired sample expression for IL-33 and TLSP (both, p = 0.046) were higher compared the effusion and serum in chronic SOM group. Conclusions: IL-33 produces inflammatory cytokines, such as IL-1b, IL-6, TNF-α, IL-10, IL-4, and TGF-β, which through nucleus into cytoplasm causing inflammatory responses. The present study revealed that IL-33 also produce IL-17, IL-4, IL-5, and IL-13 inflammatory factors, triggering an inflammatory response. Study reported that the combined stimulation of TSLP and IL-33 elicits an approximately 10-fold increase in cytokine production, when compared to the stimulation of IL-33 alone. This suggests that IL-33 and TLSP may be the primary cytokines involved in Th1/Th2 inflammatory responses in chronic SOM.

Cytokine IL-33 Secretory otitis media Th1/Th2 response Thymic stromal lymphopoietin

SCI

英语

第一 ; 通讯

Cultivating Fund Project of Shenzhen People's Hospital[SYKYPY201933]

Immunology

Immunology

WOS:000921237900029

SAGE PUBLICATIONS INC

2-s2.0-85131186691

Scopus

1.Department of Otorhinolaryngology Head and Neck Surgery,Shenzhen people’s Hospital,The Second Clinical Medical College,Jinan University,The First Affiliated Hospital,Southern University of Science and Technology,Guangdong,China
2.Department of Otorhinolaryngology Head and Neck Surgery,The First Affiliated Hospital of Jinan University,Guangdong,China
3.Department of Otorhinolaryngology Head and Neck Surgery,Hainan General Hospital,Hainan,China
4.Department of Radiology,Shenzhen People’s Hospital,The Second Clinical Medical College,Jinan University,The First Affiliated Hospital,Southern University of Science and Technology,Guangdong,China

Liao，Zhifang,Tu，Bo,Sun，Liang,et al. Interleukin-33 and thymic stromal lymphopoietin are primary cytokines involved in the Th1/Th2 inflammatory response in chronic secretory otitis media[J]. European Journal of Inflammation,2022,17.

Liao，Zhifang,Tu，Bo,Sun，Liang,Dong，Chang,Jiang，Hongyan,&Hu，Genwen.(2022).Interleukin-33 and thymic stromal lymphopoietin are primary cytokines involved in the Th1/Th2 inflammatory response in chronic secretory otitis media.European Journal of Inflammation,17.

Liao，Zhifang,et al."Interleukin-33 and thymic stromal lymphopoietin are primary cytokines involved in the Th1/Th2 inflammatory response in chronic secretory otitis media".European Journal of Inflammation 17(2022).