同时对单细胞基因组和转录组构库及测序的方法基于单细胞整合基因组学的测序方法及应用

本发明提供了一种同时对单细胞基因组和转录组进行高通量文库构建及测序的方法。本发明还提供了一种基于单细胞整合基因组学(SCIG)的测序方法及其应用。本发明提供的SCIG方案能够实现在单次实验中同时得到一个单细胞的基因组和转录组，获得单个细胞中遗传和表观遗传信息，并进行综合分析，全方位，多层面的展示该细胞的状态；这些优势使得SCIG在鉴定单细胞的特性和共性方面具有优越性。

The invention provides a method for high-throughput library construction and sequencing for both a single cell genome and transcriptome, and further provides a sequencing method based on the single cell integrated genomics (SCIG), and application of the sequencing method. Through the SCIG scheme provided by the invention, both the single cell genome and transcriptome can be obtained in a single experiment, and genetic and epigenetic information in a single cell can be obtained for comprehensive analysis, so as to achieve all-dimensional and multi-level state display of the single cell; therefore, the SCIG has superiority in detection of the general and unique characteristics of the single cell.