Deformable mirror based optimal PSF engineering for 3D super-resolution imaging

Fu, Shuang1,2; Li, Mengfan1,2; Zhou, Lulu1,2; He, Yingchuan1,2; Liu, Xin3; Hao, Xiang3; Li, Yiming1,2

2022-06-15

10.1364/OL.460949

OPTICS LETTERS   影响因子和分区

0146-9592

1539-4794

Point spread function (PSF) engineering is an important technique to encode the properties (e.g., 3D positions, color, and orientation) of a single molecule in the shape of the PSF, often with the help of a programmable phase modulator. A deformable mirror (DM) is currently the most widely used phase modulator for fluorescence detection as it shows negligible photon loss. However, it relies on careful calibration for precise wavefront control. Therefore, design of an optimal PSF not only relies on the theoretical calculation of the maximum information content, but also the physical behavior of the phase modulator, which is often ignored during the optimization process. Here, we develop a framework for PSF engineering which could generate a device specific optimal PSF for 3D super-resolution imaging using a DM. We use our method to generate two types of PSFs with depths of field comparable to the widely used astigmatism and tetrapod PSFs, respectively. We demonstrate the superior performance of the DM specific optimal PSF over the conventional astigmatism and tetrapod PSF both theoretically and experimentally. (C) 2022 Optica Publishing Group

SCI ; EI

英语

第一 ; 通讯

Shandong Key Research and Development Program[2021CXGC010212] ; Science, Technology and Innovation Commission of Shenzhen Municipality[KQTD20200820113012029] ; Guangdong Natural Science Foundation[2020A1515110380] ; Guangdong Provincial Key Laboratory of Advanced Biomaterials[2022B1212010003]

Optics

Optics

WOS:000812307400024

Optica Publishing Group

20222512261759

Adaptive optics ; Deformation ; Delta modulation ; Mirrors ; Optical resolving power ; Phase modulation

Light/Optics:741.1 ; Optical Devices and Systems:741.3

PHYSICS

Web of Science

1.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Peoples R China
2.Southern Univ Sci & Technol, Guangdong Prov Key Lab Adv Biomat, Shenzhen 518055, Peoples R China
3.Zhejiang Univ, State Key Lab Modern Opt Instrumentat, Coll Opt Sci & Technol, Hangzhou 310027, Peoples R China

Fu, Shuang,Li, Mengfan,Zhou, Lulu,et al. Deformable mirror based optimal PSF engineering for 3D super-resolution imaging[J]. OPTICS LETTERS,2022,47(12):3031-3034.

Fu, Shuang.,Li, Mengfan.,Zhou, Lulu.,He, Yingchuan.,Liu, Xin.,...&Li, Yiming.(2022).Deformable mirror based optimal PSF engineering for 3D super-resolution imaging.OPTICS LETTERS,47(12),3031-3034.

Fu, Shuang,et al."Deformable mirror based optimal PSF engineering for 3D super-resolution imaging".OPTICS LETTERS 47.12(2022):3031-3034.