Adipose mesenchymal stem cell sheets-derived extracellular vesicles-microRNA-10b promote skin wound healing by elevating expression of CDK6

Liao, Xin1; Yan, Fei2; Hu, Sean1; Mu, Jing1; Li, Siqiaozhi1; He, Yixuan1; Tang, Manshu3; Chen, Junhui4; Yu, Li2; Sun, Jia1,4

2022-05-01

10.1016/j.bioadv.2022.212781

BIOMATERIALS ADVANCES   影响因子和分区

2772-9508

Application of adipose-derived mesenchymal stromal cells (AMSCs)-derived extracellular vesicles (EVs) in skin wound healing has been documented. In this study, we investigated the therapeutic potential of AMSCs-derived EVs in skin wound healing through delivery of microRNA-10b (miR-10b). HaCaT cells were treated with H2O2 to establish the skin wound cell models. Next, the binding affinity between miR-194, PEA15, and CDK6 was identified. Additionally, EVs were isolated from the culture medium of AMSC sheets, followed by incubation with H2O2-treated HaCaT cells to detect cell proliferation, migration, and apoptosis using gain- or loss-of-function experiments. Lastly, the mice skin wound models were also established to assess skin wound healing ability. miR-10b was down-regulated in the skin trauma models and enriched in the EVs of AMSC sheets. Moreover, miR-10b derived from EVs targeted PEA15 to promote CDK6 expression, thereby stimulating the proliferation and migration of H2O2-damaged HaCaT cells but inhibiting apoptosis. In vivo experiments further ascertained the therapeutic functionality of AMSC sheets-derived EVs-miR-10b. In summary, AMSC sheets-derived EVs carrying miR-10b promoted CDK6 expression to intensify skin wound healing by regulating PEA15.

Skin wound healing Adipose-derived mesenchymal stromal cell sheets Extracellular vesicles microRNA-10b Cyclin dependent kinase 6 Phosphoprotein enriched in astrocytes 15

SCI ; EI

英语

通讯

Materials Science

Materials Science, Biomaterials

WOS:000806683400004

ELSEVIER

Web of Science

1.Shenzhen Beike Biotechnol Res Inst, 59,Gaoxin South 9th Rd, Shenzhen 518057, Guangdong, Peoples R China
2.Southern Univ Sci & Technol, Affiliated Hosp 1, Jinan Univ,Clin Med Coll 2, Shenzhen Peoples Hosp,Dept Plast & Aesthet Surg, Shenzhen 518055, Peoples R China
3.Shenzhen Toyon Biotechnol Co Ltd, Shenzhen 518057, Peoples R China
4.Peking Univ, Intervent & Cell Therapy Ctr, Shenzhen Hosp, Shenzhen 518057, Peoples R China

Liao, Xin,Yan, Fei,Hu, Sean,et al. Adipose mesenchymal stem cell sheets-derived extracellular vesicles-microRNA-10b promote skin wound healing by elevating expression of CDK6[J]. BIOMATERIALS ADVANCES,2022,136.

Liao, Xin.,Yan, Fei.,Hu, Sean.,Mu, Jing.,Li, Siqiaozhi.,...&Sun, Jia.(2022).Adipose mesenchymal stem cell sheets-derived extracellular vesicles-microRNA-10b promote skin wound healing by elevating expression of CDK6.BIOMATERIALS ADVANCES,136.

Liao, Xin,et al."Adipose mesenchymal stem cell sheets-derived extracellular vesicles-microRNA-10b promote skin wound healing by elevating expression of CDK6".BIOMATERIALS ADVANCES 136(2022).