Perfluorooctane Sulfonamide (PFOSA) Induces Cardiotoxicity via Aryl Hydrocarbon Receptor Activation in Zebrafish

Chen, Honghong1,2; Qiu, Wenhui1,2; Yang, Xuanjun3,4; Chen, Fangyi5; Chen, Jiaying5; Tang, Liang6; Zhong, Hanbing3; Magnuson, Jason T.7; Zheng, Chunmiao1; Xu, Elvis Genbo8

2022-06-21

10.1021/acs.est.1c08875

ENVIRONMENTAL SCIENCE & TECHNOLOGY   影响因子和分区

0013-936X

1520-5851

Perfluorooctane sulfonamide (PFOSA), a precursor of perfluorooctanesulfonate (PFOS), is widely used during industrial processes, though little is known about its toxicity, particularly to early life stage organisms that are generally sensitive to xenobiotic exposure. Here, following exposure to concentrations of 0.01, 0.1, 1, 10, and 100 mu g/L PFOSA, transcriptional, morphological, physiological, and biochemical assays were used to evaluate the potential effects on aquatic organisms. The top Tox functions in exposed zebrafish were related to cardiac diseases predicted by Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, and Ingenuity Pathway Analysis (IPA) analysis. Consistent with impacts predicted by transcriptional changes, abnormal cardiac morphology, disordered heartbeat signals, as well as reduced heart rate and cardiac output were observed following the exposure of 0.1, 1, 10, or 100 mu g/L PFOSA. Furthermore, these PFOSA-induced cardiac effects were either prevented or alleviated by supplementation with an aryl hydrocarbon receptor (AHR) antagonist or ahr2-morpholino knock-down, uncovering a seminal role of AHR in PFOSA-induced cardiotoxicity. Our results provide the first evidence in fish that PFOSA can impair proper heart development and function and raises concern for PFOSA analogues in the natural environment.

perfluorooctane sulfonamide cardiotoxicity heart dysfunctions aryl hydrocarbon receptor morpholino knock-down

SCI ; EI

英语

NI论文

第一 ; 通讯

National Natural Science Foundation of China[42077223] ; National Key Research and Development Program of China[2021YFA1202500] ; Shenzhen Science and Technology Innovation Committee["JCYJ20190809164201686","KCXFZ20201221173410029","KQTD2016022619584022"] ; Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control[2017B030301012] ; Guangdong Basic and Applied Basic Research Foundation[2020A1515010891]

Engineering ; Environmental Sciences & Ecology

Engineering, Environmental ; Environmental Sciences

WOS:000815664600001

AMER CHEMICAL SOC

20222512248279

Aquatic organisms ; Gene Ontology ; Genes ; Heart ; Hydrocarbons

Biological Materials and Tissue Engineering:461.2 ; Genetic Engineering:461.8.1 ; Marine Science and Oceanography:471 ; Organic Compounds:804.1

ENVIRONMENT/ECOLOGY

Web of Science

1.Southern Univ Sci & Technol, Sch Publ Hlth & Emergency Management, Shenzhen 518055, Peoples R China
2.Southern Univ Sci & Technol, Sch Environm Sci & Engn, State Environm Protect Key Lab Integrated Surface, Shenzhen 518055, Peoples R China
3.Southern Univ Sci & Technol, Sch Life Sci, Shenzhen 518055, Peoples R China
4.Univ Macau, Inst Chinese Med Sci, Macau 999078, Peoples R China
5.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Peoples R China
6.Shanghai Univ, Sch Environm & Chem Engn, Shanghai 200444, Peoples R China
7.Univ Stavanger, Dept Chem Biosci & Environm Engn, N-4021 Stavanger, Norway
8.Univ Southern Denmark, Dept Biol, DK-5230 Odense, Denmark

Chen, Honghong,Qiu, Wenhui,Yang, Xuanjun,et al. Perfluorooctane Sulfonamide (PFOSA) Induces Cardiotoxicity via Aryl Hydrocarbon Receptor Activation in Zebrafish[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2022,56(12):8438-8448.

Chen, Honghong.,Qiu, Wenhui.,Yang, Xuanjun.,Chen, Fangyi.,Chen, Jiaying.,...&Xu, Elvis Genbo.(2022).Perfluorooctane Sulfonamide (PFOSA) Induces Cardiotoxicity via Aryl Hydrocarbon Receptor Activation in Zebrafish.ENVIRONMENTAL SCIENCE & TECHNOLOGY,56(12),8438-8448.

Chen, Honghong,et al."Perfluorooctane Sulfonamide (PFOSA) Induces Cardiotoxicity via Aryl Hydrocarbon Receptor Activation in Zebrafish".ENVIRONMENTAL SCIENCE & TECHNOLOGY 56.12(2022):8438-8448.