南方科技大学知识苑(SUSTech KC): Structural basis of Tom20 and Tom22 cytosolic domains as the human TOM complex receptors

题名	Structural basis of Tom20 and Tom22 cytosolic domains as the human TOM complex receptors
作者	Su，Jiayue 1; Liu，Desheng 1; Yang，Fan 1; Zuo，Mei Qing 2; Li，Chang 1; Dong，Meng Qiu 2; Sun，Shan 1; Sui，Sen Fang 1,3
通讯作者	Sun，Shan; Sui，Sen Fang
发表日期	2022-06-28
DOI	10.1073/pnas.2200158119
发表期刊	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 影响因子和分区
ISSN	0027-8424
EISSN	1091-6490
卷号	119 期号:26
摘要	Mitochondrial preproteins synthesized in cytosol are imported into mitochondria by a multisubunit translocase of the outer membrane (TOM) complex. Functioned as the receptor, the TOM complex components, Tom 20, Tom22, and Tom70, recognize the presequence and further guide the protein translocation. Their deficiency has been linked with neurodegenerative diseases and cardiac pathology. Although several structures of the TOM complex have been reported by cryoelectron microscopy (cryo-EM), how Tom22 and Tom20 function as TOM receptors remains elusive. Here we determined the structure of TOM core complex at 2.53 Å and captured the structure of the TOM complex containing Tom22 and Tom20 cytosolic domains at 3.74 Å. Structural analysis indicates that Tom20 and Tom22 share a similar three-helix bundle structural feature in the cytosolic domain. Further structure-guided biochemical analysis reveals that the Tom22 cytosolic domain is responsible for binding to the presequence, and the helix H1 is critical for this binding. Altogether, our results provide insights into the functional mechanism of the TOM complex recognizing and transferring preproteins across the mitochondrial membrane.
关键词	cryo-EM mitochondria TOM complex Tom20 Tom22
相关链接	[Scopus记录]
收录类别	SCI
语种	英语
重要成果	NI论文
学校署名	通讯
资助项目	National Basic Research Program[2017YFA0504601] ; National Natural Science Foundation of China["32071192","91954118"]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000854978700011
出版者	NATL ACAD SCIENCES
ESI学科分类	BIOLOGY & BIOCHEMISTRY;CLINICAL MEDICINE;MULTIDISCIPLINARY;PLANT & ANIMAL SCIENCE;ENVIRONMENT/ECOLOGY;SOCIAL SCIENCES, GENERAL;MICROBIOLOGY;ECONOMICS BUSINESS;IMMUNOLOGY;MATERIALS SCIENCE;MATHEMATICS;SPACE SCIENCE;MOLECULAR BIOLOGY & GENETICS;PHARMACOLOGY & TOXICOLOGY;CHEMISTRY;PSYCHIATRY/PSYCHOLOGY;NEUROSCIENCE & BEHAVIOR;PHYSICS;GEOSCIENCES;AGRICULTURAL SCIENCES;ENGINEERING
Scopus记录号	2-s2.0-85132626081
来源库	Scopus
引用统计	被引频次[WOS]：26
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/352486
专题	生命科学学院_生物系生命科学学院
作者单位	1.State Key Laboratory of Membrane Biology,Beijing Advanced Innovation Center for Structural Biology,Beijing Frontier Research Center for Biological Structure,School of Life Sciences,Tsinghua University,100084,China 2.National Institute of Biological Sciences,102206,China 3.Department of Biology,Southern University of Science and Technology,Shenzhen,518055,China
通讯作者单位	生物系; 生命科学学院
推荐引用方式 GB/T 7714	Su，Jiayue,Liu，Desheng,Yang，Fan,et al. Structural basis of Tom20 and Tom22 cytosolic domains as the human TOM complex receptors[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2022,119(26).
APA	Su，Jiayue.,Liu，Desheng.,Yang，Fan.,Zuo，Mei Qing.,Li，Chang.,...&Sui，Sen Fang.(2022).Structural basis of Tom20 and Tom22 cytosolic domains as the human TOM complex receptors.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,119(26).
MLA	Su，Jiayue,et al."Structural basis of Tom20 and Tom22 cytosolic domains as the human TOM complex receptors".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 119.26(2022).