南方科技大学知识苑(SUSTech KC): Triphenyl phosphate (TPP) promotes hepatocyte toxicity via induction of endoplasmic reticulum stress and inhibition of autophagy flux

题名	Triphenyl phosphate (TPP) promotes hepatocyte toxicity via induction of endoplasmic reticulum stress and inhibition of autophagy flux
作者	Li, Miaoran 1,2,3; Liu, Gang 1; Yuan, Li-Xia 4; Yang, Jing 2,3 ; Liu, Jing 2,3 ; Li, Zhijie 2,3 ; Yang, Chuanbin 2,3; Wang, Jigang 2,3,4,5
通讯作者	Yang, Chuanbin; Wang, Jigang
发表日期	2022-09-20
DOI	10.1016/j.scitotenv.2022.156461
发表期刊	SCIENCE OF THE TOTAL ENVIRONMENT 影响因子和分区
ISSN	0048-9697
EISSN	1879-1026
卷号	840
摘要	Triphenyl phosphate (TPP), a commonly used organophosphate flame retardant, is frequently found in environmental and biota samples, indicating widespread human exposure. Recent studies have shown that TPP causes hepatotoxicity, but the underlying cellular mechanisms are not fully elucidated. Here, by using normal hepatocyte AML12 cells as a model, we showed that TPP induced apoptotic cell death. RNA sequencing analyses revealed that differentially expressed genes induced by TPP were related to endoplasmic reticulum (ER) stress and autophagy. Immunostaining and western blot results further confirmed that TPP activated ER stress. Interestingly, though TPP increased LC3-II, a canonical marker for autophagy, TPP inhibited autophagy flux rather than induced autophagy. Interestingly, TPPinduced ER stress facilitated autophagy flux inhibition and apoptosis. Furthermore, inhibition of autophagy aggravated, and activation of autophagy attenuated apoptosis induced by TPP. Collectively, these results uncovered that ER stress and autophagy flux inhibition were responsible for TPP-induced apoptosis in mouse hepatocytes. Thus, our foundlings provided novel insight into the potential mechanisms of TPP-induced hepatocyte toxicity.
关键词	Triphenyl phosphate Hepatoxicity ER stress Autophagy Apoptosis
相关链接	[来源记录]
收录类别	SCI ; EI
语种	英语
学校署名	通讯
资助项目	Shenzhen Science and Technology Innovation Commission["JCYJ20210324115800001","JCYJ20210324114014039"] ; National Key Research and Development Program of China[2020YFA0908000] ; Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine[ZYYCXTD-C-20200 2]
WOS研究方向	Environmental Sciences & Ecology
WOS类目	Environmental Sciences
WOS记录号	WOS:000816884800010
出版者	ELSEVIER
ESI学科分类	ENVIRONMENT/ECOLOGY
来源库	Web of Science
引用统计	被引频次[WOS]：16
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/353394
专题	南方科技大学第一附属医院
作者单位	1.Southern Med Univ, Nanfang Hosp, Dept Rehabil Med, Guangzhou 510515, Peoples R China 2.Jinan Univ, Clin Med Coll 2, Shenzhen Peoples Hosp, Dept Geriatr Med, Shenzhen 518020, Peoples R China 3.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen 518020, Peoples R China 4.Southern Med Univ, Guangdong Prov Key Lab Chinese Med Pharmaceut, Sch Tradit Chinese Med, Guangzhou 510515, Peoples R China 5.Chinese Acad Chinese Med Sci, Inst Chinese Mat Med, Artemisinin Res Ctr, Beijing 100700, Peoples R China
第一作者单位	南方科技大学第一附属医院
通讯作者单位	南方科技大学第一附属医院
推荐引用方式 GB/T 7714	Li, Miaoran,Liu, Gang,Yuan, Li-Xia,et al. Triphenyl phosphate (TPP) promotes hepatocyte toxicity via induction of endoplasmic reticulum stress and inhibition of autophagy flux[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2022,840.
APA	Li, Miaoran.,Liu, Gang.,Yuan, Li-Xia.,Yang, Jing.,Liu, Jing.,...&Wang, Jigang.(2022).Triphenyl phosphate (TPP) promotes hepatocyte toxicity via induction of endoplasmic reticulum stress and inhibition of autophagy flux.SCIENCE OF THE TOTAL ENVIRONMENT,840.
MLA	Li, Miaoran,et al."Triphenyl phosphate (TPP) promotes hepatocyte toxicity via induction of endoplasmic reticulum stress and inhibition of autophagy flux".SCIENCE OF THE TOTAL ENVIRONMENT 840(2022).