Comparing the toxicity of iodinated X-ray contrast media on eukaryote- and prokaryote-based quantified microarray assays

Yang，Heyun1; Li，Xiaoliang1; Zheng，Xing1; Zhi，Hegang2; Tang，Gang3; Ke，Yanchun4; Liu，Bao1; Ma，Hao1

2022-07-01

10.1016/j.ecoenv.2022.113678

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY   影响因子和分区

0147-6513

1090-2414

This study compared the toxicity mechanisms of four X-ray-based iodinated contrast media (ICM) on Escherichia coli (E. coli) and yeast microarray assays, aiming to determine the diverse toxicity mechanisms among different exposed organisms and the relationship between toxicity and their physical and chemical characteristics. The conventional phenotypic endpoint cytotoxicity and the change in reactive oxygen species (ROS) level were employed in conjunction with toxicogenomics to quantify changes in the gene/protein biomarker level in the regulation of different damage/repair pathways. The results showed that molecular toxicity endpoints, named transcriptional effect level index (TELI) and protein effect level index (PELI) for E. coli and yeast, respectively, correlated well with the phenotypic endpoints. Temporal altered gene/protein expression profiles revealed dynamic and complex damage/toxic mechanisms. In particular, compared with E. coli cells, yeast cells exposed to ICM exhibited significantly higher stress intensity and diverse stress types, resulting in stress or damage to the organism. The toxic mechanisms of ICM are concentration/property-dependent and relevant to the cellular structure and defense systems in prokaryotes and eukaryotes. In particular, the toxicity of ionic ICM is higher than that of non-ionic ICM, and eukaryotes are more susceptible than prokaryotes to ICM exposure.

ICM Toxicity mechanism Phenotypic and molecular toxicity endpoints Prokaryotes Eukaryotes

SCI

英语

其他

National Natural Science Foundation of China["52100104","52170053","51878555"] ; Scientific Research Projects of Shaanxi Education Department[19JS049]

Environmental Sciences & Ecology ; Toxicology

Environmental Sciences ; Toxicology

WOS:000832716100003

ACADEMIC PRESS INC ELSEVIER SCIENCE

ENVIRONMENT/ECOLOGY

2-s2.0-85131054863

Web of Science

1.State Key Laboratory of Eco-hydraulics in North west Arid Region,Xi'an University of Technology,Xi'an,710048,China
2.College of Agricultural and Environmental Sciences,University of California,Davis,95616,United States
3.State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control,Southern University of Science and Technology,Shenzhen,518055,China
4.Aerospace Kaitian environmental technology co. ltd,Changsha,410100,China

Yang，Heyun,Li，Xiaoliang,Zheng，Xing,et al. Comparing the toxicity of iodinated X-ray contrast media on eukaryote- and prokaryote-based quantified microarray assays[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2022,240.

Yang，Heyun.,Li，Xiaoliang.,Zheng，Xing.,Zhi，Hegang.,Tang，Gang.,...&Ma，Hao.(2022).Comparing the toxicity of iodinated X-ray contrast media on eukaryote- and prokaryote-based quantified microarray assays.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,240.

Yang，Heyun,et al."Comparing the toxicity of iodinated X-ray contrast media on eukaryote- and prokaryote-based quantified microarray assays".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 240(2022).