Epidemiology and Genomic Characteristics of Bloodstream Infection Caused by Carbapenem-Resistant Klebsiella pneumoniae With Decreased Susceptibility to Aztreonam/Avibactam in China

Yu, Wei1; Shen, Ping1; Chen, Yunbo1; Zhou, Kai2,3; Chi, Xiaohui1; Xiao, Yonghong1

2022-06-22

10.3389/fcimb.2022.926209

Frontiers in Cellular and Infection Microbiology   影响因子和分区

2235-2988

Aztreonam/avibactam (AZA), as one of the novel beta-lactamases and beta-lactamase inhibitor combinations, is considered to be a promising option for bloodstream infection (BSI) of carbapenem-resistant Klebsiella pneumoniae (CR-Kp). However, decreased susceptibility of AZA activity in Enterobacterales has been reported. The aim of this study was to identify the mechanisms of BSI CR-Kp with decreased susceptibility of AZA (minimal inhibitory concentration above 16/4 mg/L) (AZAH-Kp). Nine BSI AZAH-Kp isolates were screened from 317 CR-Kp isolates in Blood Bacterial Resistant Investigation Collaborative System (BRICS) program. Whole genome sequencing, bioinformatics analysis, and the relative expression of bla(KPC), ompK35, and ompK37 were explored for CR-Kp with decreased susceptibility to AZA. The results revealed that elevated inhibitory concentration of AZA has emerged in CR-Kp before previous clinical exposure. In addition, decreased AZA susceptibility was associated with higher KPC expression and changes in OmpK35-37.

carbapenem-resistant Klebsiella pneumoniae aztreonam avibactam bla(KPC) membrane porin mutation

SCI

英语

其他

Immunology ; Microbiology

Immunology ; Microbiology

WOS:000822591900001

FRONTIERS MEDIA SA

Web of Science

1.Zhejiang Univ, Sch Med, Hangzhou, Peoples R China
2.Jinan Univ, Shenzhen Peoples Hosp, Shenzhen Inst Resp Dis, Clin Med Coll 2, Shenzhen, Peoples R China
3.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen Peoples Hosp, Shenzhen, Peoples R China

Yu, Wei,Shen, Ping,Chen, Yunbo,et al. Epidemiology and Genomic Characteristics of Bloodstream Infection Caused by Carbapenem-Resistant Klebsiella pneumoniae With Decreased Susceptibility to Aztreonam/Avibactam in China[J]. Frontiers in Cellular and Infection Microbiology,2022,12.

Yu, Wei,Shen, Ping,Chen, Yunbo,Zhou, Kai,Chi, Xiaohui,&Xiao, Yonghong.(2022).Epidemiology and Genomic Characteristics of Bloodstream Infection Caused by Carbapenem-Resistant Klebsiella pneumoniae With Decreased Susceptibility to Aztreonam/Avibactam in China.Frontiers in Cellular and Infection Microbiology,12.

Yu, Wei,et al."Epidemiology and Genomic Characteristics of Bloodstream Infection Caused by Carbapenem-Resistant Klebsiella pneumoniae With Decreased Susceptibility to Aztreonam/Avibactam in China".Frontiers in Cellular and Infection Microbiology 12(2022).