Ethylene treatment in studying leaf senescence in Arabidopsis

Li，Zhonghai1; Guo，Hongwei1,2

2018

Arabidopsis Ethylene Inhibitor Leaf senescence

英语

Ministry of Agriculture of the People's Republic of China[2010ZX08010-002];National Natural Science Foundation of China[91017010];

Plant leaf senescence is the final process of leaf development that involves the mobilization of nutrients from old leaves to newly growing tissues. Leaf senescence involves a coordinated action at the cellular and organism levels under the control of a highly regulated genetic program. However, leaf senescence is also influenced by multiple internal and environmental signals that are integrated into the age information. Among these internal factors, the simple gaseous phytohormone ethylene is well-known to be an important endogenous modulator of plant leaf senescence and fruit ripening. Ethylene and its biosynthetic precursor ACC (1-aminocyclopropane-l-carboxylic acid) as well as inhibitors of ethylene biosynthesis aminoethoxyvinylglycine (AVG) and action (AgNO) or the inhibitor of ethylene perception 1-MCP (1-methylcyclopropene) have been widely used by the research community of plant leaf senescence. However, until now, no systemically experimental method about the usage of ethylene in studying the molecular mechanisms of leaf senescence in Arabidopsis is available. Here, we provide detailed methods for exogenous application of ethylene and its inhibitors in studying Arabidopsis leaf senescence, which has been successfully used in our laboratory.

10.1007/978-1-4939-7672-0_8

Methods in Molecular Biology

1744

105-112

1064-3745

通讯

2-s2.0-85041749389

Scopus

1.The State Key Laboratory of Protein and Plant Gene Research,College of Life Sciences,Peking-Tsinghua Center of Life Sciences,Peking University,Beijing,China
2.Department of Biology,South University of Science and Technology of China,Shenzhen,China

Li，Zhonghai,Guo，Hongwei. Ethylene treatment in studying leaf senescence in Arabidopsis. 2018-01-01.