南方科技大学知识苑(SUSTech KC): Dual-CRISPR/Cas12a-Assisted RT-RAA for Ultrasensitive SARS-CoV-2 Detection on Automated Centrifugal Microfluidics

题名	Dual-CRISPR/Cas12a-Assisted RT-RAA for Ultrasensitive SARS-CoV-2 Detection on Automated Centrifugal Microfluidics
作者	Chen, Yong1,2 ; Zong, Nan1,2 ; Ye, Feidi 3; Mei, Yixin1 ; Qu, Jiuxin 3; Jiang, Xingyu1,2
通讯作者	Qu, Jiuxin; Jiang, Xingyu
发表日期	2022-07-01
DOI	10.1021/acs.analchem.2c00638
发表期刊	ANALYTICAL CHEMISTRY 影响因子和分区
ISSN	0003-2700
EISSN	1520-6882
卷号	94页码:9603-9609
摘要	The clustered regularly interspaced short palindromic repeats (CRISPR)-based nucleic acid detection can be combined with recombinase-aided amplification (RAA) to enable rapid, accurate, and early detection of SARS-CoV-2. Current CRISPR-based approaches to detecting viral nucleic acid typically require immense manual operations to transfer RPA amplicons for CRISPR detection or suffer from compromised sensitivity by mixing the competing RPA amplification and CRISPR detection. Here, we develop dual-CRISPR/Cas12a-assisted RT-RAA assay and a "sample-to-answer " centrifugal microfluidic platform that can automatically detect 1 copy/mu L of the SARS-CoV-2 within 30 min. This chip separates the amplification (RAA) from detection (CRISPR), such that sensitivity is maximized and the time consumption is decreased by a factor of 3. For the 26 positive and 8 negative clinical SARS-CoV-2 samples, this automated centrifugal microfluidics achieved 100% accuracy compared to the gold standard RT-PCR technique. This point-of-care test, with the advantages of being one-step, automated, rapid, and sensitive, will have a significant potential for clinical diagnosis and disease prevention.
相关链接	[来源记录]
收录类别	SCI ; EI
语种	英语
重要成果	NI论文
学校署名	第一 ; 通讯
资助项目	National Key R&D Program of China[2018YFA0902600] ; National Natural Science Foundation of China["22104048","21535001","81730051","1761142006"] ; Guangdong Province["2022B1212010003","2019ZT08Y191","2020A1515010586"] ; Department of Education of Guangdong Province[2019KQNCX130] ; Shenzhen Key Laboratory of Smart Healthcare Engineering[ZDSYS20200811144003009] ; Tencent Foundation["2018YFA0902600","22104048","21535001"] ; [KQTD20190929172743294] ; [JCYJ20200109141231365] ; [JCYJ20190809144005609]
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000826302200001
出版者	AMER CHEMICAL SOC
EI入藏号	20223012418006
EI主题词	Automation ; Centrifugation ; Coronavirus ; Microfluidics ; Polymerase chain reaction
EI分类号	Medicine and Pharmacology:461.6 ; Microfluidics:632.5.1 ; Automatic Control Principles and Applications:731 ; Biochemistry:801.2 ; Chemical Operations:802.3
ESI学科分类	CHEMISTRY
来源库	Web of Science
引用统计	被引频次[WOS]：46
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/356216
专题	工学院_生物医学工程系南方科技大学第一附属医院南方科技大学第二附属医院
作者单位	1.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen Key Lab Smart Healthcare Engn, Shenzhen 518055, Guangdong, Peoples R China 2.Southern Univ Sci & Technol, Guangdong Prov Key Lab Adv Biomat, Shenzhen 518055, Guangdong, Peoples R China 3.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Dept Clin Lab, Natl Clin Res Ctr Infect Dis,Hosp 2, Shenzhen 518114, Guangdong, Peoples R China
第一作者单位	生物医学工程系; 南方科技大学
通讯作者单位	南方科技大学第二附属医院; 南方科技大学第一附属医院; 生物医学工程系; 南方科技大学
第一作者的第一单位	生物医学工程系
推荐引用方式 GB/T 7714	Chen, Yong,Zong, Nan,Ye, Feidi,et al. Dual-CRISPR/Cas12a-Assisted RT-RAA for Ultrasensitive SARS-CoV-2 Detection on Automated Centrifugal Microfluidics[J]. ANALYTICAL CHEMISTRY,2022,94:9603-9609.
APA	Chen, Yong,Zong, Nan,Ye, Feidi,Mei, Yixin,Qu, Jiuxin,&Jiang, Xingyu.(2022).Dual-CRISPR/Cas12a-Assisted RT-RAA for Ultrasensitive SARS-CoV-2 Detection on Automated Centrifugal Microfluidics.ANALYTICAL CHEMISTRY,94,9603-9609.
MLA	Chen, Yong,et al."Dual-CRISPR/Cas12a-Assisted RT-RAA for Ultrasensitive SARS-CoV-2 Detection on Automated Centrifugal Microfluidics".ANALYTICAL CHEMISTRY 94(2022):9603-9609.