Regulation of uric acid and glyoxylate metabolism by UgmR protein in Pseudomonas aeruginosa

Xu，Xuejie1; Yan，Yunfang1; Huang，Jiadai2; Zhang，Zihao1; Wang，Zhihan3; Wu，Min3; Liang，Haihua1,4

2022-07-01

10.1111/1462-2920.16088

ENVIRONMENTAL MICROBIOLOGY   影响因子和分区

1462-2912

1462-2920

The opportunistic pathogen Pseudomonas aeruginosa has evolved several systems to adapt to complex environments. The GntR family proteins play important roles in the regulation of metabolic processes and bacterial pathogenesis. In this study, we uncovered that the gene clusters of PA1513-PA1518 and PA1498-PA1502 in P. aeruginosa are required for uric acid and glyoxylate metabolism respectively. We also identified a GntR family regulator UgmR that is involved in regulation of uric acid and glyoxylate metabolism. Promoter activity measurement and biochemical assays revealed that the UgmR directly represses the transcriptional activity of PA1513-PA1518 and PA1498-PA1502, and this inhibition was relieved by the addition of uric acid. Importantly, further experiments showed that UgmR also participates in the glyoxylate shunt. Collectively, these findings contribute to a better understanding of the UgmR factor involved in uric acid and glyoxylate metabolism, which provide insights into the complex metabolic pathways in P. aeruginosa.

SCI

英语

通讯

National Natural Science Foundation of China[32170188 31870060] ; Shaanxi Science and Technology Innovation Team[2019TD-016]

Microbiology

Microbiology

WOS:000827609300028

WILEY

ENVIRONMENT/ECOLOGY

2-s2.0-85134225272

Web of Science

1.Key Laboratory of Resources Biology and Biotechnology in Western China,Ministry of Education,College of Life Sciences,Northwest University,Xi'an,Shaanxi,China
2.Department of Biomedical Sciences,City University of Hong Kong,Hong Kong
3.Department of Biomedical Sciences,School of Medicine and Health Sciences,University of North Dakota,Grand Forks,United States
4.College of Medicine,Southern University of Science and Technology,Shenzhen,China

Xu，Xuejie,Yan，Yunfang,Huang，Jiadai,et al. Regulation of uric acid and glyoxylate metabolism by UgmR protein in Pseudomonas aeruginosa[J]. ENVIRONMENTAL MICROBIOLOGY,2022,24(7):3242-3255.

Xu，Xuejie.,Yan，Yunfang.,Huang，Jiadai.,Zhang，Zihao.,Wang，Zhihan.,...&Liang，Haihua.(2022).Regulation of uric acid and glyoxylate metabolism by UgmR protein in Pseudomonas aeruginosa.ENVIRONMENTAL MICROBIOLOGY,24(7),3242-3255.

Xu，Xuejie,et al."Regulation of uric acid and glyoxylate metabolism by UgmR protein in Pseudomonas aeruginosa".ENVIRONMENTAL MICROBIOLOGY 24.7(2022):3242-3255.