MiR-138-5p Targets MACF1 to Aggravate Aging-related Bone Loss

Chen, Zhihao1,2,7; Huai, Ying1,2; Chen, Gaoyang3; Liu, Shuyu1,2; Zhang, Yan1,2,4,5; Li, Dijie1,2,6; Zhao, Fan1,2,4,5; Chen, Xiaofeng4,5; Mao, Wenjing1,2; Wang, Xuehao1,2; Yin, Chong1,2; Yang, Chaofei1,2; Xu, Xia1,2; Ru, Kang1,2; Deng, Xiaoni1,2; Hu, Lifang1,2; Li, Yu1,2; Peng, Songlin3; Zhang, Ge6; Lin, Xiao1,2; Qian, Airong1,2

2022

10.7150/ijbs.71411

International Journal of Biological Sciences   影响因子和分区

1449-2288

Senile osteoporosis is one of the major health problems in an aging society. Decreased bone formation due to osteoblast dysfunction may be one of the causes of aging-related bone loss. With increasing evidence suggesting that multiple microRNAs (miRNAs) play important roles in osteoblast function, the relationship between miRNAs and senile osteoporosis has become a popular research topic. Previously, we confirmed that mechanoresponsive miR-138-5p negatively regulated bone anabolic action. In this study, the miR-138-5p level was found to be negatively correlated with BMD and osteogenic markers in bone specimens of senile osteoporotic patients by bioinformatic analysis and experimental verification. Furthermore, high miR-138-5p levels aggravated the decrease of aged osteoblast differentiation in vitro and led to worse bone loss in aged osteoblastic miR-138-5p transgenic mice in vivo. We also previously identified that the target of miR-138-5p, microtubule actin cross-linking factor 1 (MACF1), could attenuate senile osteoporosis. Here, miR-138-5p was demonstrated to regulate aged osteoblast differentiation by targeting MACF1. Finally, the therapeutic inhibition of miR-138-5p counteracted the decrease in bone formation and aging-related bone loss in aged mice. Overall, our results highlight the crucial roles and the molecular mechanism of miR-138-5p in aging-related bone loss and may provide a powerful therapeutic target for ameliorating senile osteoporosis.

  senile osteoporosis miR-138-5p MACF1 osteoblast differentiation aging -related bone loss

SCI

英语

通讯

Natural Science Foundation of China["32101055","82072106","31570940","81872129","81700784","81772017"] ; China Postdoctoral Science Foundation["2020M683573","2017M613196"] ; Natural Science Basic Research Plan in Shaanxi Province of China["2021JQ-128","2018JM3040"] ; Shaanxi Provincial Key RD Program["2018KWZ-10","2021SF-242"] ; Innovation Foundation for Doctor Dissertation of Northwestern Polytechnical University[CX201821]

Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics

Biochemistry & Molecular Biology ; Biology

WOS:000829372300003

IVYSPRING INT PUBL

Web of Science

1.Northwestern Polytech Univ, Lab Bone Metab, Xian Key Lab Special Med & Hlth Engn, Sch Life Sci, Xian 710072, Shaanxi, Peoples R China
2.Northwestern Polytech Univ, Key Lab Space Biosci & Biotechnol, Res Ctr Special Med & Hlth Syst Engn, NPU UAB Joint Lab Bone Metab,Sch Life Sci, Xian 710072, Shaanxi, Peoples R China
3.Jinan Univ, Sch Med, Southern Univ Sci & Technol, Dept Spine Surg,Shenzhen Peoples Hosp, Shenzhen 518020, Peoples R China
4.Xi An Jiao Tong Univ, Sch Life Sci & Technol, Minist Educ, Key Lab Biomed Informat Engn, Xian 710049, Shaanxi, Peoples R China
5.Xi An Jiao Tong Univ, Sch Life Sci & Technol, Inst Mol Genet, Xian 710049, Shaanxi, Peoples R China
6.Hong Kong Baptist Univ, Sch Chinese Med, Law Sau Fai Inst Adv Translat Med Bone & Joint Di, Hong Kong 999077, Peoples R China
7.Fourth Mil Med Univ, Xijing Hosp, Dept Gynaecol & Obstet, Xian 710032, Shaanxi, Peoples R China

Chen, Zhihao,Huai, Ying,Chen, Gaoyang,et al. MiR-138-5p Targets MACF1 to Aggravate Aging-related Bone Loss[J]. International Journal of Biological Sciences,2022,18(13).

Chen, Zhihao.,Huai, Ying.,Chen, Gaoyang.,Liu, Shuyu.,Zhang, Yan.,...&Qian, Airong.(2022).MiR-138-5p Targets MACF1 to Aggravate Aging-related Bone Loss.International Journal of Biological Sciences,18(13).

Chen, Zhihao,et al."MiR-138-5p Targets MACF1 to Aggravate Aging-related Bone Loss".International Journal of Biological Sciences 18.13(2022).