| 题名 | IRSp53 promotes postsynaptic density formation and actin filament bundling |
| 作者 | |
| 通讯作者 | Kim，Eunjoon; Zhang，Mingjie |
| 发表日期 | 2022-08-01
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| DOI | |
| 发表期刊 | |
| ISSN | 0021-9525
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| EISSN | 1540-8140
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| 卷号 | 221期号:8 |
| 摘要 | IRSp53 (aka BAIAP2) is a scaffold protein that couples membranes with the cytoskeleton in actin-filled protrusions such as filopodia and lamellipodia. The protein is abundantly expressed in excitatory synapses and is essential for synapse development and synaptic plasticity, although with poorly understood mechanisms. Here we show that specific multivalent interactions between IRSp53 and its binding partners PSD-95 or Shank3 drive phase separation of the complexes in solution. IRSp53 can be enriched to the reconstituted excitatory PSD (ePSD) condensates via bridging to the core and deeper layers of ePSD. Overexpression of a mutant defective in the IRSp53/PSD-95 interaction perturbs synaptic enrichment of IRSp53 in mouse cortical neurons. The reconstituted PSD condensates promote bundled actin filament formation both in solution and on membranes, via IRSp53-mediated actin binding and bundling. Overexpression of mutants that perturb IRSp53-actin interaction leads to defects in synaptic maturation of cortical neurons. Together, our studies provide potential mechanistic insights into the physiological roles of IRSp53 in synapse formation and function. |
| 相关链接 | [Scopus记录] |
| 收录类别 | |
| 语种 | 英语
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| 重要成果 | NI论文
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| 学校署名 | 通讯
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| 资助项目 | National Natural Science Foundation of China[82188101];Human Frontier Science Program[RGP0020/2019];
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| WOS研究方向 | Cell Biology
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| WOS类目 | Cell Biology
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| WOS记录号 | WOS:000868315400001
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| 出版者 | |
| ESI学科分类 | MOLECULAR BIOLOGY & GENETICS
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| Scopus记录号 | 2-s2.0-85134427019
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| 来源库 | Scopus
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| 引用统计 |
被引频次[WOS]：11
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| 成果类型 | 期刊论文 |
| 条目标识符 | http://sustech.caswiz.com/handle/2SGJ60CL/359548 |
| 专题 | 生命科学学院 |
| 作者单位 | 1.Division of Life Science,State Key Laboratory of Molecular Neuroscience,Hong Kong University of Science and Technology,Hong Kong,China 2.State Key Laboratory of Genetic Engineering,School of Life Sciences,Fudan University,China 3.Center for Synaptic Brain Dysfunctions,Institute for Basic Science (IBS),South Korea 4.Department of Biological Sciences,Korea Advanced Institute of Science and Technology (KAIST),South Korea 5.School of Life Sciences,Southern University of Science and Technology,Shenzhen,China |
| 通讯作者单位 | 生命科学学院 |
| 推荐引用方式 GB/T 7714 |
Feng，Zhe,Lee，Suho,Jia，Bowen,et al. IRSp53 promotes postsynaptic density formation and actin filament bundling[J]. JOURNAL OF CELL BIOLOGY,2022,221(8).
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| APA |
Feng，Zhe,Lee，Suho,Jia，Bowen,Jian，Tao,Kim，Eunjoon,&Zhang，Mingjie.(2022).IRSp53 promotes postsynaptic density formation and actin filament bundling.JOURNAL OF CELL BIOLOGY,221(8).
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| MLA |
Feng，Zhe,et al."IRSp53 promotes postsynaptic density formation and actin filament bundling".JOURNAL OF CELL BIOLOGY 221.8(2022).
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| 条目包含的文件 | 条目无相关文件。 | |||||
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