Adhesive hydrogel wrap loaded with Netrin-1-modified adipose-derived stem cells: An effective approach against periarterial inflammation after endovascular intervention

Jiang，Yihong1; Cai，Yuting2,3; Hu，Jiateng1; Zhang，Xing1; Lei，Jiahao1; Peng，Zhaoxi1; Huang，Qun1; Xu，Zhijue1; Li，Bo1; Qin，Jinbao1; Li，Weimin1; Sun，Dazhi3; Ye，Kaichuang1; Lu，Xinwu1

2022-07-22

10.3389/fbioe.2022.944435

Frontiers in Bioengineering and Biotechnology   影响因子和分区

2296-4185

2296-4185

Endovascular interventions, such as balloon dilation and stent implantation, are currently recommended as the primary treatment for patients with peripheral artery disease (PAD), greatly improving patient prognosis. However, the consequent lumen restenosis that occurs after endovascular interventions has become an important clinical problem. Inflammation has been proven to be crucial to postoperative restenosis. In previous studies we have identified that Netrin-1-modified adipose-derived stem cells (N-ADSCs) transplantation is an effective anti-inflammatory strategy to repair vascular damage. Nevertheless, it remained to be explored how one could constantly deliver N-ADSCs onto damaged arteries. Therefore, we developed an adhesive double network (DN) hydrogel wrap loaded with N-ADSCs for sustained perivascular delivery. Inspired by the adhesion mechanism of mussels, we developed an adhesive and tough polyacrylamide/calcium-alginate/reduced graphene oxide/polydopamine (PAM/CA/rGO/PDA) hydrogel. Dopamine was attached to graphene sheets and limitedly oxidized to generate free catechol groups. The hydrogel could wrap damaged arteries and induce anti-inflammatory effects through N-ADSCs. In vitro experiments demonstrated that N-ADSCs significantly promoted the M2 polarization of macrophages to anti-inflammatory phenotypes and reduced the expression of inflammatory factors. In vivo experiments in a rat carotid artery guidewire injury model showed that the adhesive hydrogel wrap loaded with N-ADSCs could significantly reduce arterial inflammation, inhibit intimal hyperplasia and improve re-endothelialization. Altogether, this newly developed N-ADSCs-loaded hydrogel wrap provides an effective slow-releasing system, which may be a promising way to prevent and treat restenosis after endovascular interventions.

adhesive hydrogel adipose-derived stem cells dopamine endovascular interventions intimal hyperplasia macrophages Netrin-1 re-endothelialization

SCI ; EI

英语

通讯

National Natural Science Foundation of China[82170509 81971758 81971712];

Biotechnology & Applied Microbiology ; Science & Technology - Other Topics

Biotechnology & Applied Microbiology ; Multidisciplinary Sciences

WOS:000837334000001

FRONTIERS MEDIA SA

20223212549317

Amines ; Diagnosis ; Diseases ; Graphene ; Hydrogels ; Macrophages ; Neurophysiology ; Pathology ; Patient treatment ; Stem cells

Biological Materials and Tissue Engineering:461.2 ; Medicine and Pharmacology:461.6 ; Immunology:461.9.1 ; Nanotechnology:761 ; Colloid Chemistry:801.3 ; Chemical Products Generally:804 ; Organic Compounds:804.1

2-s2.0-85135469690

Scopus

1.Department of Vascular Surgery,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,China
2.Department of Chemical and Biological Engineering,William Mong Institute of Nano Science and Technology,The Hong Kong University of Science and Technology,Kowloon,Hong Kong
3.Guangdong Provincial Key Laboratory of Functional Oxide Materials and Devices,Southern University of Science and Technology,Shenzhen,Guangdong,China

Jiang，Yihong,Cai，Yuting,Hu，Jiateng,et al. Adhesive hydrogel wrap loaded with Netrin-1-modified adipose-derived stem cells: An effective approach against periarterial inflammation after endovascular intervention[J]. Frontiers in Bioengineering and Biotechnology,2022,10.

Jiang，Yihong.,Cai，Yuting.,Hu，Jiateng.,Zhang，Xing.,Lei，Jiahao.,...&Lu，Xinwu.(2022).Adhesive hydrogel wrap loaded with Netrin-1-modified adipose-derived stem cells: An effective approach against periarterial inflammation after endovascular intervention.Frontiers in Bioengineering and Biotechnology,10.

Jiang，Yihong,et al."Adhesive hydrogel wrap loaded with Netrin-1-modified adipose-derived stem cells: An effective approach against periarterial inflammation after endovascular intervention".Frontiers in Bioengineering and Biotechnology 10(2022).