The Dynamic Epigenetic Study of Prostate Cancer

Prostate cancer (PCa) is the leading cancer diagnosed among men. The androgen receptor (AR) regulates downstream gene transcription after binding with its ligand in both normal and cancerous prostate cells. Here, we captured the genome conformation of both AR-inactivated and AR-activated VCaP cells using Hi-C technology to reveal the gene regulation induced by AR activation with respect to the change in 3D organization of the genome. The global conformation of the genome showed no obvious change at the compartment and Topological associating domain (TAD) levels. In contrast, the transcriptome of AR-activated VCaP cells was significantly regulated, and AR binding was greatly increased. Changes in long-range interactions were related to significantly regulated genes, and the remote regions were highly enriched with AR and other factor binding signals.

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