| 题名 | miR-133a-5p Inhibits Glioma Cell Proliferation by Regulating IGFBP3 |
| 作者 | |
| 通讯作者 | Lv, Wen |
| 发表日期 | 2022-08-02
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| DOI | |
| 发表期刊 | |
| ISSN | 1687-8450
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| EISSN | 1687-8469
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| 卷号 | 2022 |
| 摘要 | Objective. This research aims to investigate the expression of miR-133a-5p in glioma tissues and its impact on glioma cell proliferation. Methods. Fluorescence-quantitative PCR was used to detect the expression of miR-133a-5p in 25 cases of glioma and adjuncent tissues. CCK-8 and colony formation analyses were used to evaluate the impact of transfection with miR-133a-5p inhibitors or mimics on glioma cell growth and colony formation. The IGFBP3 (insulin-like growth factor-binding protein-3) and miR-133a-5p binding sites were predicted using Starbase, and the miR-133a-5p binding capacity with 3'UTR of IGFBP3 gene was determined using a luciferase gene reporter system. Following transfection with miR-133a-5p mimics or inhibitors, the IGFBP3 protein expression in glioma cells was determined by western blotting. The colony formation assay was applied to evaluate the influence of IGFBP3 overexpression on the miR-133a-5p in glioma cell proliferation. For assessment of the IGFBP3 expression in glioma tissues and prognosis, TCGA database was employed. Results. The expression of miR-133a-5p was considerably reduced in glioma tissue compared to adjuncent control tissue. In addition, miR-133a-5p expression decreased with increasing glioma malignancy. Glioma cell growth and colony formation were reduced after miR-133a-5p mimics were transfected, while transfection of miR-133a-5p inhibitors had a reverse impact. The expression of IGFBP3 was affected by miR-133a-5p by binding to its 3'UTR region. Additional study demonstrated that the overall survival (OS) of subjects with increased IGFBP3 expression was considerably lower compared to patients with decreased IGFBP3 expression. The IGFBP3 overexpression effectively counteracts the glioma cell proliferation-inhibiting impact of miR-133a-5p. Conclusion. miR-133a-5p acts as a glioma tumor suppressor gene. It reduces glioma cell proliferation by modulating IGFBP3 and could be a target for glioma therapy. |
| 相关链接 | [来源记录] |
| 收录类别 | |
| 语种 | 英语
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| 学校署名 | 通讯
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| 资助项目 | [JCYJ20210324114008023]
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| WOS研究方向 | Oncology
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| WOS类目 | Oncology
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| WOS记录号 | WOS:000840759000001
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| 出版者 | |
| 来源库 | Web of Science
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| 引用统计 |
被引频次[WOS]：3
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| 成果类型 | 期刊论文 |
| 条目标识符 | http://sustech.caswiz.com/handle/2SGJ60CL/382305 |
| 专题 | 南方科技大学第一附属医院 |
| 作者单位 | 1.Southern Med Univ, Dept Neurosurg, Shenzhen Hosp, Shenzhen, Guangdong, Peoples R China 2.Shenzhen Yantian Dists Peoples Hosp, Dept Neurosurg, Shenzhen, Guangdong, Peoples R China 3.Shenzhen Peoples Hosp, Dept Neurosurg, Shenzhen, Guangdong, Peoples R China 4.Guangdong Engn Technol Res Ctr Nervous Anat & Rela, Zhongshan, Peoples R China 5.Univ South China, Dept Neurosurg, Changsha Cent Hosp, Changsha, Hunan, Peoples R China 6.Southern Univ Sci & Technol, Affiliated Hosp 1, Dept Neurosurg, Shenzhen, Guangdong, Peoples R China |
| 通讯作者单位 | 南方科技大学第一附属医院 |
| 推荐引用方式 GB/T 7714 |
Yang, Xinzhi,Chen, Dong,Hu, Jiliang,et al. miR-133a-5p Inhibits Glioma Cell Proliferation by Regulating IGFBP3[J]. Journal of Oncology,2022,2022.
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| APA |
Yang, Xinzhi,Chen, Dong,Hu, Jiliang,Zhao, Qingsuo,Fu, Xing,&Lv, Wen.(2022).miR-133a-5p Inhibits Glioma Cell Proliferation by Regulating IGFBP3.Journal of Oncology,2022.
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| MLA |
Yang, Xinzhi,et al."miR-133a-5p Inhibits Glioma Cell Proliferation by Regulating IGFBP3".Journal of Oncology 2022(2022).
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| 条目包含的文件 | 条目无相关文件。 | |||||
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