| 题名 | Virulence and biofilm inhibition of 3-methoxycinnamic acid against Agrobacterium tumefaciens |
| 作者 | |
| 通讯作者 | Li, Jun-Jian; Jia, Ai-Qun |
| 发表日期 | 2022-08-01
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| DOI | |
| 发表期刊 | |
| ISSN | 1364-5072
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| EISSN | 1365-2672
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| 摘要 | Aims In the current study the anti-virulence and anti-biofilm activities of the cinnamic acid derivative, 3-methoxycinnamic acid, was investigated against Agrobacterium tumefaciens. Methods and Results Based on the disc diffusion test and beta-galactosidase activity assay, 3-methoxycinnamic acid was shown to interfere with the quorum sensing (QS) system of A. tumefaciens. Crystal violet staining assay, phenol-sulfuric acid method, Bradford protein assay and confocal laser scanning microscopy (CLSM) revealed that the biofilm formation of A. tumefaciens was inhibited after the treatment of 3-methoxycinnamic acid. Employing high-performance liquid chromatography (HPLC) analysis of culture supernatant revealed that the production of 3-oxo-octanoylhomoserine lactone (3-oxo-C8-HSL) decreased concentration-dependently after treatment with 3-methoxycinnamic acid. Swimming and chemotaxis assays also indicated that 3-methoxycinnamic acid had a good effect on reducing the motility and chemotaxis of A. tumefaciens. In addition, the RT-qPCR, molecular docking and simulations further demonstrated that 3-methoxycinnamic acid could competitively inhibit the binding of 3-oxo-C8-HSL to TraR and down-regulate virulence-related genes. Conclusions 3-Methoxycinnamic acid is proved to have good anti-virulence and anti-biofilm activities against A. tumefaciens. Significance and Impact of the Study This is the first study that investigates the anti-virulence and anti-biofilm activities of 3-methoxycinnamic acid against A. tumefaciens. With its potential QS-related virulence and biofilm inhibitory activities, 3-methoxycinnamic acid is expected to be developed as a potent pesticide or adjuvant for the prevention and treatment of crown gall caused by A. tumefaciens. |
| 关键词 | |
| 相关链接 | [来源记录] |
| 收录类别 | |
| 语种 | 英语
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| 学校署名 | 其他
|
| 资助项目 | Jiangsu Youth Talent Promotion Project[TJ-2021-066]
; General Projects of Natural Science Research in Universities of Jiangsu Province[20KJB180019]
; Natural Science Foundation of Hainan Province["221CXTD434","319QN165"]
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| WOS研究方向 | Biotechnology & Applied Microbiology
; Microbiology
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| WOS类目 | Biotechnology & Applied Microbiology
; Microbiology
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| WOS记录号 | WOS:000842824600001
|
| 出版者 | |
| ESI学科分类 | BIOLOGY & BIOCHEMISTRY
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| 来源库 | Web of Science
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| 引用统计 |
被引频次[WOS]：4
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| 成果类型 | 期刊论文 |
| 条目标识符 | http://sustech.caswiz.com/handle/2SGJ60CL/382581 |
| 专题 | 南方科技大学医学院 |
| 作者单位 | 1.Hainan Univ, Sch Pharmaceut Sci, Haikou 570228, Hainan, Peoples R China 2.Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, Haikou, Hainan, Peoples R China 3.Xuzhou Univ Technol, Sch Food & Biol Engn, Xuzhou, Jiangsu, Peoples R China 4.Southern Univ Sci & Technol, Sch Med, Shenzhen, Peoples R China 5.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Dept Bioinformat & Biol Stat, Shanghai, Peoples R China 6.Zhongjing Res & Industrializat Inst Chinese Med, Nayang, Peoples R China 7.Hainan Univ, One Hlth Inst, Haikou, Hainan, Peoples R China |
| 推荐引用方式 GB/T 7714 |
Pan, Deng,Wang, Xing-Yun,Zhou, Jin-Wei,et al. Virulence and biofilm inhibition of 3-methoxycinnamic acid against Agrobacterium tumefaciens[J]. JOURNAL OF APPLIED MICROBIOLOGY,2022.
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| APA |
Pan, Deng.,Wang, Xing-Yun.,Zhou, Jin-Wei.,Yang, Liang.,Khan, Abbas.,...&Jia, Ai-Qun.(2022).Virulence and biofilm inhibition of 3-methoxycinnamic acid against Agrobacterium tumefaciens.JOURNAL OF APPLIED MICROBIOLOGY.
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| MLA |
Pan, Deng,et al."Virulence and biofilm inhibition of 3-methoxycinnamic acid against Agrobacterium tumefaciens".JOURNAL OF APPLIED MICROBIOLOGY (2022).
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| 条目包含的文件 | 条目无相关文件。 | |||||
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