南方科技大学知识苑(SUSTech KC): Cryo-EM structure studies of the human VPS10 domain-containing receptor SorCS3

题名	Cryo-EM structure studies of the human VPS10 domain-containing receptor SorCS3
作者	Dong，Fengquan 1,2; Wu，Cang 3; Jiang，Weipeng 4; Zhai，Maocai 2; Li，Haiying 1; Zhai，Liting 5; Zhang，Xi 6,7
通讯作者	Li，Haiying; Zhai，Liting; Zhang，Xi
发表日期	2022-10-08
DOI	10.1016/j.bbrc.2022.07.009
发表期刊	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 影响因子和分区
ISSN	0006-291X
EISSN	1090-2104
卷号	624页码:89-94
摘要	The human VPS10 domain-containing receptor SorCS3 belongs to the Vps10p-domain receptor family and is an important receptor for regulating normal cellular functions via protein sorting. Here, we determined the cryo-EM structure of the full-length SorCS3 receptor and further found that there were at least three distinct conformations (monomer, M-shaped dimer and N-shaped dimer) of SorCS3 in the apo state. The differences between the two dimer conformations were caused by PKD1-2 assembly. In contrast to its homologous proteins, the conserved residues GLN198, ARG678, TYR430, GLU1020 and ASP1024 may be key points for its dimerization and for protein/polypeptide binding. These results showed the structural details of apo-SorCS3, which provides a foundation for elucidating the mechanism of protein sorting.
关键词	Apo-SorCS3 Conformation change Cryo-EM structure Dimerization
相关链接	[Scopus记录]
收录类别	SCI
语种	英语
学校署名	其他
资助项目	Medical Scientific Reuter Foundation of Guangdong Province of China[A2021333] ; Shenzhen Science and Technology Innovation Commission Project[JCYJ20190808120613189] ; Shenzhen Pea-cock Program-Project Development Fund[20190904141C]
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
WOS记录号	WOS:000852817200014
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
ESI学科分类	BIOLOGY & BIOCHEMISTRY
Scopus记录号	2-s2.0-85135813394
来源库	Scopus
引用统计	被引频次[WOS]：1
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/382611
专题	南方科技大学
作者单位	1.The University of Hong Kong–Shenzhen Hospital,Shenzhen,Guangdong Province,518000,China 2.Shenzhen University General Hospital,Shenzhen,Guangdong Province,518000,China 3.Southern University of Science and Technology,Shenzhen,Guangdong Province,518055,China 4.South China Hospital of Shenzhen University,Shenzhen,Guangdong Province,518111,China 5.Stanford University,Stanford,CA 94305,United States 6.Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences,Shenzhen,Guangdong Province,518055,China 7.Shenzhen People's Second Hospital,Shenzhen,Guangdong Province,518000,China
推荐引用方式 GB/T 7714	Dong，Fengquan,Wu，Cang,Jiang，Weipeng,et al. Cryo-EM structure studies of the human VPS10 domain-containing receptor SorCS3[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2022,624:89-94.
APA	Dong，Fengquan.,Wu，Cang.,Jiang，Weipeng.,Zhai，Maocai.,Li，Haiying.,...&Zhang，Xi.(2022).Cryo-EM structure studies of the human VPS10 domain-containing receptor SorCS3.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,624,89-94.
MLA	Dong，Fengquan,et al."Cryo-EM structure studies of the human VPS10 domain-containing receptor SorCS3".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 624(2022):89-94.