Quantitative Measurement and Analysis in a Synthetic Pattern Formation Multicellular System

Fu, Xiongfei1; Huang, Wei2,3; Sun, L; Shou, W

2014

10.1007/978-1-4939-0554-6_10

Methods in Molecular Biology   影响因子和分区

1064-3745

Pattern formation has been studied for more than a century in biology. In recent years there are increasing interests in studying it using bacteria and synthetic biology tools to program intercellular communication and cellular response to environment. Quantitative measurement is critical to dissect the interplay between the synthetic gene circuits with underline cellular processes and verify the mechanism determining the pattern formation. Here, we describe simple optical setups for quantitative measurements of the cell density and growth and spatial-temporal dynamic characterization of E. coli pattern formation in soft agar plates.

Pattern formation Synthetic biology Quantitative biology Quorum sensing Time-lapse imaging Optical density

BKCI-S ; BSCI

英语

其他

Biochemistry & Molecular Biology

Biochemical Research Methods ; Biochemistry & Molecular Biology

WOS:000338666100011

HUMANA PRESS INC

Web of Science

1.Univ Hong Kong, Dept Phys, Pokfulam, Hong Kong, Peoples R China
2.South Univ Sci & Technol China, Dept Biol, Shenzhen, Peoples R China
3.Univ Hong Kong, Dept Biochem, Pokfulam, Hong Kong, Peoples R China

Fu, Xiongfei,Huang, Wei,Sun, L,et al. Quantitative Measurement and Analysis in a Synthetic Pattern Formation Multicellular System[J]. Methods in Molecular Biology,2014,1151:137-149.

Fu, Xiongfei,Huang, Wei,Sun, L,&Shou, W.(2014).Quantitative Measurement and Analysis in a Synthetic Pattern Formation Multicellular System.Methods in Molecular Biology,1151,137-149.

Fu, Xiongfei,et al."Quantitative Measurement and Analysis in a Synthetic Pattern Formation Multicellular System".Methods in Molecular Biology 1151(2014):137-149.