| 题名 | Ulinastatin alleviates pulmonary edema by reducing pulmonary permeability and stimulating alveolar fluid clearance in a rat model of acute lung injury |
| 作者 | |
| 通讯作者 | Jiang, Yuan-xu |
| 发表日期 | 2022-08-01
|
| DOI | |
| 发表期刊 | |
| ISSN | 2008-3866
|
| EISSN | 2008-3874
|
| 卷号 | 25期号:8 |
| 摘要 | Objective(s): Previous studies have shown that ulinastatin (UTI) alleviates pulmonary edema in acute lung injury (ALI) caused by lipopolysaccharide (LPS), although the mechanism behind this action is uncertain. This research aimed to identify the fundamental mechanism by which UTI alleviates pulmonary edema. Materials and Methods: We established a model of acute lung injury (ALI) in rats by using LPS as the inciting agent.The control, LPS, and LPS+UTI groups were each comprised of a specific number of randomly selected Wistar rats. We evaluated lung injury and determined pulmonary edema. The concentrations of TNF-alpha, IL-10 and IL-6 in BALF and the expression levels of alpha 1Na, k-ATPase, 01Na, K-AtPase, alpha-ENaC, 0-ENaC, gamma-ENaC, Zonula occludens (ZO)-1, Occludin, Caludin-5, PI3K, Akt, TLR4, MyD88 and NF-KBwere identified in lung tissues. Results: The presence of UTI was associated with a reduction in lung pathological alterations, lung injury scores, the lung W/D ratio, and MPO activity, in addition to the improved gas exchange (P<0.01). Furthermore, UTI alleviated EB leakage and stimulated AFC (P<0.01). Importantly, UTI increased the expression of ZO-1, Occludin, Caludin-5, alpha 1Na, K-ATPase, 01Na, K-AtPase, alpha-ENaC, 0-ENaC, and gamma-ENaC (P<0.01). Furthermore, UTI inhibited the inflammatory response, enhanced the expression of PI3K and Akt and hindered TLR4, MyD88, and NF-KB expression (P<0.01) in lung tissues. Conclusion: Our results demonstrated that UTI attenuated pulmonary edema by reducing pulmonary permeability and promoting AFC through inhibiting the inflammatory response, and the mechanism is related to promoting PI3K/Akt signaling pathways and suppressing TLR4/MyD88/NF-KB signaling pathways. |
| 关键词 | |
| 相关链接 | [来源记录] |
| 收录类别 | |
| 语种 | 英语
|
| 学校署名 | 第一
; 通讯
|
| 资助项目 | Shenzhen Key Medical Discipline Construction Fund[SZXK044]
; Sanming Project of Medicine in Shenzhen[SZSM202011021]
|
| WOS研究方向 | Research & Experimental Medicine
; Pharmacology & Pharmacy
|
| WOS类目 | Medicine, Research & Experimental
; Pharmacology & Pharmacy
|
| WOS记录号 | WOS:000844170200007
|
| 出版者 | |
| 来源库 | Web of Science
|
| 引用统计 |
被引频次[WOS]：4
|
| 成果类型 | 期刊论文 |
| 条目标识符 | http://sustech.caswiz.com/handle/2SGJ60CL/394267 |
| 专题 | 南方科技大学第一附属医院 |
| 作者单位 | 1.Southern Univ Sci & Technol, Jinan Univ, Affiliated Hosp 1, Clin Med Coll 2,Shenzhen Peoples Hosp,Dept Anesth, Shenzhen 518020, Guangdong, Peoples R China 2.Shenzhen Peoples Hosp, Dept Crit Care Med, Shenzhen 518020, Guangdong, Peoples R China |
| 第一作者单位 | 南方科技大学第一附属医院 |
| 通讯作者单位 | 南方科技大学第一附属医院 |
| 第一作者的第一单位 | 南方科技大学第一附属医院 |
| 推荐引用方式 GB/T 7714 |
Jiang, Yuan-xu,Huang, Ze-wei. Ulinastatin alleviates pulmonary edema by reducing pulmonary permeability and stimulating alveolar fluid clearance in a rat model of acute lung injury[J]. Iranian Journal of Basic Medical Sciences,2022,25(8).
|
| APA |
Jiang, Yuan-xu,&Huang, Ze-wei.(2022).Ulinastatin alleviates pulmonary edema by reducing pulmonary permeability and stimulating alveolar fluid clearance in a rat model of acute lung injury.Iranian Journal of Basic Medical Sciences,25(8).
|
| MLA |
Jiang, Yuan-xu,et al."Ulinastatin alleviates pulmonary edema by reducing pulmonary permeability and stimulating alveolar fluid clearance in a rat model of acute lung injury".Iranian Journal of Basic Medical Sciences 25.8(2022).
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| 条目包含的文件 | 条目无相关文件。 | |||||
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