南方科技大学知识苑(SUSTech KC): Knockdown of ARL5B Induces Mitochondrial-mediated Apoptosis and Inhibits Glycolysis in Breast Cancer Cells by Activating MDA5 Signaling

题名	Knockdown of ARL5B Induces Mitochondrial-mediated Apoptosis and Inhibits Glycolysis in Breast Cancer Cells by Activating MDA5 Signaling
作者	Zhang, Lei ; Hu, Xuqiao; Wu, Huaiyu; Tian, Hongtian; Zeng, Jieying; Song, Di; Yang, Keen; Chen, Jing; Xu, Jinfeng; Dong, Fajin
通讯作者	Xu, Jinfeng; Dong, Fajin
发表日期	2022
DOI	10.2174/1568009622666220511112538
发表期刊	CURRENT CANCER DRUG TARGETS 影响因子和分区
ISSN	1568-0096
EISSN	1873-5576
卷号	22 期号:10
摘要	Aim: Mitochondria are essential for energy metabolism in the tumor microenvironment and the survival of cancer cells. Background: ADP-ribosylation factor-like GTPase 5b (ARL5B) has been found to be associated with mitochondrial dysfunction and breast cancer (BC) progression, but the underlying mechanism needs to be further understood. Objective: We investigated the effects of ARL5B on the apoptosis and glycolysis of breast cancer cells and its underlying mechanisms. Methods: Quantitative reverse transcription-PCR (qRT-PCR) and western blot assays were used to detect the expression of ARL5B in breast cancer tissues and cells. An ARL5B loss-of-function assay was performed to verify its role in BC development. Results: ARL5B was upregulated in breast cancer tissues and cell lines. ARL5B knockdown induced apoptosis and activated the mitochondrial pathway in breast cancer cells. Interestingly, the inhibition of ARL5B repressed the aerobic glycolysis of breast cancer cells. The role of ARL5B in breast cancer cells was exerted by mediating the activation of viral RNA sensor MDA5-evoked signaling. Silencing ARL5B triggered MDA5 signaling by upregulating the key proteins involved in the MDA5 pathway. Importantly, MDA5 silencing reversed the effects of ARL5B knockdown on mitochondrial-mediated apoptosis and glycolysis, whereas poly (I:C), as a ligand for MDA5, further enhanced ARL5B knockdown-facilitated mitochondrial apoptosis and the inhibition of glycolysis. Conclusion: The knockdown of ARL5B activated MDA5 signaling and thus led to the enhanced mitochondrial-mediated apoptosis and glycolysis inhibition in breast cancer cells. Our study suggested that ARL5B might be a potential therapy target for BC.
关键词	Energy metabolism apoptosis ARL5B breast cancer mitochondria cytochrome C
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	第一 ; 通讯
资助项目	[Natural Science Foundation of Shenzhen][JCYJ201908061-51807192]
WOS研究方向	Oncology
WOS类目	Oncology
WOS记录号	WOS:000863838700005
出版者	BENTHAM SCIENCE PUBL LTD
ESI学科分类	PHARMACOLOGY & TOXICOLOGY
来源库	Web of Science
引用统计	被引频次[WOS]：0
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/405976
专题	南方科技大学第一附属医院
作者单位	Jinan Univ, Shenzhen Peoples Hosp, Affiliated Hosp 1,Southern Univ Sci & Technol, Dept Ultrasound,Clin Med Coll 2, Shenzhen 518020, Guangdong, Peoples R China
第一作者单位	南方科技大学第一附属医院
通讯作者单位	南方科技大学第一附属医院
第一作者的第一单位	南方科技大学第一附属医院
推荐引用方式 GB/T 7714	Zhang, Lei,Hu, Xuqiao,Wu, Huaiyu,et al. Knockdown of ARL5B Induces Mitochondrial-mediated Apoptosis and Inhibits Glycolysis in Breast Cancer Cells by Activating MDA5 Signaling[J]. CURRENT CANCER DRUG TARGETS,2022,22(10).
APA	Zhang, Lei.,Hu, Xuqiao.,Wu, Huaiyu.,Tian, Hongtian.,Zeng, Jieying.,...&Dong, Fajin.(2022).Knockdown of ARL5B Induces Mitochondrial-mediated Apoptosis and Inhibits Glycolysis in Breast Cancer Cells by Activating MDA5 Signaling.CURRENT CANCER DRUG TARGETS,22(10).
MLA	Zhang, Lei,et al."Knockdown of ARL5B Induces Mitochondrial-mediated Apoptosis and Inhibits Glycolysis in Breast Cancer Cells by Activating MDA5 Signaling".CURRENT CANCER DRUG TARGETS 22.10(2022).