Engineered Spore-Forming Bacillus as a Microbial Vessel for Long-Term DNA Data Storage

Liu, Feng1; Li, Jiashu1; Zhang, Tongzhou1; Chen, Jun1,2; Ho, Chun Loong1,2

2022-09-01

10.1021/acssynbio.2c00291

ACS Synthetic Biology   影响因子和分区

2161-5063

DNA data storage technology may supersede conventional chip or magnetic data storage medium, providing long-term stability, high density, and sustainable storage. Due to its error-correcting capability, DNA data stored in living organisms exhibits high fidelity in information replication. Here we report the development of a Bacillus chassis integrated with an inducible artificially assembled bacterial chromosome to facilitate random data access. We generated three sets of data in the form of DNA sequences using a rudimentary coding system accessible by the regulatory promoter. Sporulated Bacillus harboring the genes were used for long-term storage, where viability assays of spores were subjected to harsh environmental stresses to evaluate the data storage stability. The data accuracy remained above 99% after high temperature and oxidative stress treatment, whereas UV irradiation treatment provided above 96% accuracy. The developed Bacillus chassis and artificial chromosome facilitate the long-term storage of larger datum volume by using other DNA digital encoding and decoding programs.

synthetic biology DNA storage Bacillus spore artificial chromosome

SCI

英语

第一 ; 通讯

Ministry of Science and Technology, National Key RD Program[2018YF0902604] ; National Natural Science Foundation of China?s Research Fund for International Young Scientists[22050410270] ; Shenzhen Special Fund for Innovation and Entrepreneurship of Overseas High-level Talents Peacock Team[KQTD20170810111314625] ; Guangdong Innovative and Entrepreneurial Research Team Program[2019ZT08Y191]

Biochemistry & Molecular Biology

Biochemical Research Methods

WOS:000861617900001

AMER CHEMICAL SOC

Web of Science

1.Southern Univ Sci & Technol SUSTech, Dept Biomed Engn, Shenzhen 518055, Peoples R China
2.Chinese Acad Sci, Shenzhen Inst Adv Technol SIAT, Shenzhen Inst Synthet Biol, Shenzhen 518055, Peoples R China

Liu, Feng,Li, Jiashu,Zhang, Tongzhou,et al. Engineered Spore-Forming Bacillus as a Microbial Vessel for Long-Term DNA Data Storage[J]. ACS Synthetic Biology,2022.

Liu, Feng,Li, Jiashu,Zhang, Tongzhou,Chen, Jun,&Ho, Chun Loong.(2022).Engineered Spore-Forming Bacillus as a Microbial Vessel for Long-Term DNA Data Storage.ACS Synthetic Biology.

Liu, Feng,et al."Engineered Spore-Forming Bacillus as a Microbial Vessel for Long-Term DNA Data Storage".ACS Synthetic Biology (2022).